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| Status |
Public on Sep 08, 2014 |
| Title |
Effect of Titanium dioxide nanoparticles on Escherichia coli K12 MG1655 genes expression |
| Platform organisms |
Escherichia coli O157:H7 str. EDL933; Escherichia coli CFT073; Escherichia coli O157:H7 str. Sakai; Escherichia coli str. K-12 substr. MG1655 |
| Sample organism |
Escherichia coli str. K-12 substr. MG1655 |
| Experiment type |
Expression profiling by array
|
| Summary |
In the present study, we investigated the transcriptional expression patterns of the model strain E. coli exposed to titanium dioxide nanoparticles (NP-TiO2), under dark conditions by using a microarray. Expression profiles were compared to unexposed E.coli and ratio of expression were analysed.
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| Overall design |
Cell exposure to NP-TiO2 was conducted in 10 mM NaCl, E. coli bacterial suspension and NP-TiO2 stock suspension (or mQ water for the control) were added to the NaCl solution to obtain final concentrations of 10E7 cells/ml and 100 mg/l of TiO2 nanoparticles. The flasks were then incubated at 20°C on a dark rotary shaker for 5 h. Exposed NP-TiO2: 4 biological replicats. Control: 4 biological replicats.
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| Contributor(s) |
Sohm B, Pagnout C |
| Citation(s) |
25346333 |
| |
| Submission date |
Mar 17, 2014 |
| Last update date |
Dec 08, 2014 |
| Contact name |
Bénédicte SOHM |
| E-mail(s) |
sohm5@univ-lorraine.fr
|
| Phone |
0033372748942
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| Organization name |
CNRS
|
| Lab |
UMR 7360
|
| Street address |
8 rue du Gal Delestraint
|
| City |
METZ |
| ZIP/Postal code |
57070 |
| Country |
France |
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| Platforms (1) |
| GPL13359 |
Agilent-020097 E. coli Gene Expression Microarray (Probe Name version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA241458 |
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