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| Status |
Public on Aug 13, 2015 |
| Title |
Cytomegalovirus promotes maintenance and growth of glioblastoma stem cells [Human gene expression] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Primary human GBM stem like cells were infected with HCMV TR strain (MOI=1) and treated with IE siRNA (a combination of oligos targeting IE1 and IE2 HCMV genes)
72 hours following siRNA treatment, RNA was harvested using Qiagen and divided equally for profiling using Affymetrix arrays and HCMV arrays.
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| |
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| Overall design |
Primary human glioma stem cells were maintained in neurosphere growth conditions, treated with IE1 and control siRNA for 48-72h prior to RNA harvesting. RNA was hybridized to Affymetrix Genechips.
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| Contributor(s) |
Soroceanu L, Ghanny S |
| Citation(s) |
26239477 |
| Submission date |
Apr 10, 2014 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Saleena Ghanny |
| E-mail(s) |
ghannysa@njms.rutgers.edu
|
| Organization name |
Rutgers University
|
| Street address |
185 South Orange Ave
|
| City |
Newark |
| ZIP/Postal code |
07103 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE56752 |
Human Cytomegalovirus in Glioblastoma Stemness--Results from Human, Mouse and HCMV DNA arrays |
|
| Relations |
| BioProject |
PRJNA244438 |
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