|
| Status |
Public on Aug 13, 2015 |
| Title |
0609T |
| Sample type |
RNA |
| |
|
| Source name |
Primary GSC Infected TR treated control siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Brain Tumor
cell type: glioblastoma
|
| Treatment protocol |
40 nM final conc of control siRNA or IE siRNA (2 duplexes targeting IE1 and IE2 were added to triplicate wells. RNA harvested 72h following treatment
|
| Growth protocol |
Primary GBM lines were grown as neurospheres in Neural Basal Media + 20ng/ml FGF and EGF.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Qiagen Rneasy kit, according to the manufacturer's instructions
|
| Label |
Biotin
|
| Label protocol |
The Ambion WT Expression Kit with accompanying protocol was used to label the samples
|
| |
|
| Hybridization protocol |
The Affymetrix Genechip WT Terminal Labeling and Hybridization protocol was used
|
| Scan protocol |
Chips were scanned using the Affymetrix 7G Plus scanner and Command Console Software
|
| Description |
GBM0609T
Infected with HCMV TR strain
|
| Data processing |
Data was processed by importing CEL files into Partek Software and RMA normalization was performed
|
| |
|
| Submission date |
Apr 10, 2014 |
| Last update date |
Aug 13, 2015 |
| Contact name |
Saleena Ghanny |
| E-mail(s) |
ghannysa@njms.rutgers.edu
|
| Organization name |
Rutgers University
|
| Street address |
185 South Orange Ave
|
| City |
Newark |
| ZIP/Postal code |
07103 |
| Country |
USA |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE56715 |
Cytomegalovirus promotes maintenance and growth of glioblastoma stem cells [Human gene expression] |
| GSE56752 |
Human Cytomegalovirus in Glioblastoma Stemness--Results from Human, Mouse and HCMV DNA arrays |
|
