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| Status |
Public on Sep 30, 2014 |
| Title |
Whole transcriptome responses among females of the filariasis and arbovirus vector mosquito Culex pipiens implicate TGF-β signaling and chromatin modification as key drivers of diapause induction |
| Platform organism |
Culex quinquefasciatus |
| Sample organisms |
Culex pipiens; Culex quinquefasciatus |
| Experiment type |
Expression profiling by array
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| Summary |
Investigation of gene expression level differences in Culex pipiens (SB) and C. quinquefasciatus (JHB) at three time points (8, 16, and 24 hours post-exposure) during the early pupal state in standard (25°C; 16 h light/8 h dark) and diapause-inducing (18°C; 8 h light/16 h dark) conditions.
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| Overall design |
A forty-eight chip study (4 Expr12x135K slides) using cDNA from total RNA collected from two species in the Culex pipiens complex at 3 time-points during the early pupal stage to study gene expression differences between standard (25°C; 16 h light/8 h dark) and diapause-inducing (18°C; 8 h light/16 h dark) conditions, and between Culex pipiens (diapause) and C. quinquefasciatus (no diapause). Each chip measures the expression level of 18,692 protein coding genes, with 3 probes per gene and two-fold technical redundancy. Each of the 12-plex slides was used for one of four biological replicates. Probes were designed using the C.quinquefasciatus CpipJ1.2 geneset in VectorBase.
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| Contributor(s) |
Hickner PV, Mori A, Zeng E, Tan J, Severson DW |
| Citation(s) |
25634120 |
| |
| Submission date |
Apr 10, 2014 |
| Last update date |
Feb 06, 2015 |
| Contact name |
David W Severson |
| Organization name |
University of Notre Dame
|
| Department |
Biological Sciences
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| Lab |
David W Severson
|
| Street address |
44 Galvin Life Sciences
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| City |
Notre Dame |
| State/province |
IN |
| ZIP/Postal code |
46556 |
| Country |
USA |
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| Platforms (1) |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA244427 |
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