|
| Status |
Public on Mar 17, 2015 |
| Title |
Determination of decay rates across the transcriptome and identification of controlling features |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this work, we determine andenylated and total mRNA decay rates and correlate them with codon optimality. We conclude that optimality is a major contributor to mRNA stability in budding yeast.
|
| |
|
| Overall design |
mRNA profiling was done on 10 time points using two different library prep methods and sequenced using a paired end protocol on an Illumina HiSeq2000 instrument. A biological duplicate was performed.
|
| |
|
| Contributor(s) |
Presnyak V, Olson S, Graveley B, Coller J |
| Citation(s) |
25768907 |
| Submission date |
May 07, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Jeffery Michael Coller |
| E-mail(s) |
jmc71@case.edu
|
| Phone |
216-368-0299
|
| Organization name |
Case Western Reserve University
|
| Department |
Center for RNA Molecular Biology
|
| Street address |
2109 Adelbert Dr.
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44106 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
|
| Samples (40)
|
|
| Relations |
| BioProject |
PRJNA246375 |
| SRA |
SRP041746 |
Less...
More...