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| Status |
Public on Dec 31, 2014 |
| Title |
MiR-133b regulates the expression of the actin protein TAGLN2 during oocyte growth and maturation: a potential target for infertility therapy |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
In this study, the expression of miRNAs in metaphase I (MI) oocytes treated with or without insulin-like growth factor 1 (IGF-1) was observed by microRNA microarray analysis. Results show that 145 miRNAs were up-regulated and 200 miRNAs were down-regulated in MI oocytes after IGF-1 treatment. MiR-133b, which was up-regulated more than 30-fold, was chosen for further research.
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| Overall design |
The isolated MI oocytes were cultured at 37°C and 5% CO2 in G-2TM PLUS (Vitrolife, Sweden) treated with (45 oocytes) or without (62 oocytes) 200 ng/ml IGF-1 for 24 h (Peprotech, USA), then covered with ovoil (Vitrolife, Sweden). After 24 h, all oocytes were stored at 80°C until RNA was extracted. The average age of patients without IGF-1 treatment was 31.66 ± 3.90. The average age of patients with IGF-1 treatment was 31.25 ± 3.31. No apparent bias was detected between these two groups.
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| Contributor(s) |
Guo XH, Cheng XL |
| Citation(s) |
24959893 |
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| Submission date |
May 14, 2014 |
| Last update date |
Aug 16, 2019 |
| Contact name |
Chenglai Xia |
| E-mail(s) |
xiachenglai@126.com
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| Organization name |
Affiliated Foshan Maternity & Chlid Healthcare Hospital, Southern Medical University
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| Street address |
Renming Xi 11#
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| City |
Foshan |
| ZIP/Postal code |
528000 |
| Country |
China |
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| Platforms (1) |
| GPL18116 |
Exiqon miRCURY LNA microRNA array, 7th generation REV [miRBase v18.0, condensed Probe_ID version] |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA247460 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE57657.txt.gz |
70.9 Kb |
(ftp)(http) |
TXT |
| GSE57657_RAW.tar |
1.9 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
| Processed data are available on Series record |
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