|
| Status |
Public on Dec 31, 2014 |
| Title |
MI IGF-1 oocytes |
| Sample type |
RNA |
| |
|
| Source name |
MI oocytes
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: female
average age: 31.26 years old
treatment: IGF-1
|
| Treatment protocol |
The isolated MI oocytes were cultured at 37°C and 5% CO2 in G-2TM PLUS (Vitrolife, Sweden) treated with (45 oocytes) or without (62 oocytes) 200 ng/ml IGF-1 for 24 h (Peprotech, USA), then covered with ovoil (Vitrolife, Sweden). After 24 h, all oocytes were stored at 80°C until RNA was extracted. The average age of patients without IGF-1 treatment was 31.66 ± 3.90. The average age of patients with IGF-1 treatment was 31.25 ± 3.31. No apparent bias was detected between these two groups.
|
| Growth protocol |
After the informed consent approval was obtained, the oocytes were collected from the patients who were explicitly informed about the research aims, their rights and interests in the research. From April to July 2013, a total of 107 oocytes at MI stage were obtained from patients who received intra-cytoplasmic sperm injection (ICSI) treatments at the Reproductive Medical Center of the Third Affiliated Hospital of Guangzhou Medical University.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Denuded oocytes were stored in Trizol at -80°C prior to RNA purification. RNA from 45 oocytes in each treatment as above was pooled. To satisfy the minimum requirement of 250 ng RNA for microarray analysis, 300–400 ng RNA were used for each analysis.
|
| Label |
Hy3
|
| Label protocol |
The miRCURY™ Hy3™/Hy5™ Power labeling kit (Exiqon, Vedbaek, Denmark) was used according to the manufacturer’s guideline for miRNA labelling
|
| |
|
| Hybridization protocol |
The Hy3TM-labeled samples were hybridized on the miRCURYTM LNA Array (v.16.0) (Exiqon) according to array manual
|
| Scan protocol |
The slides were scanned using the Axon GenePix 4000B microarray scanner (Axon Instruments, Foster City, CA).
|
| Data processing |
Scanned images were then imported into GenePix Pro 6.0 software (Axon) for grid alignment and data extraction. Replicated miRNAs were averaged and miRNAs that intensities>=50 in all samples were chosen for calculating normalization factor. Expressed data were normalized using the Median normalization. After normalization, differentially expressed miRNAs with statistical significance were identified through Volcano Plot filtering.
|
| |
|
| Submission date |
May 14, 2014 |
| Last update date |
Dec 31, 2014 |
| Contact name |
Chenglai Xia |
| E-mail(s) |
xiachenglai@126.com
|
| Organization name |
Affiliated Foshan Maternity & Chlid Healthcare Hospital, Southern Medical University
|
| Street address |
Renming Xi 11#
|
| City |
Foshan |
| ZIP/Postal code |
528000 |
| Country |
China |
| |
|
| Platform ID |
GPL18116 |
| Series (1) |
| GSE57657 |
MiR-133b regulates the expression of the actin protein TAGLN2 during oocyte growth and maturation: a potential target for infertility therapy |
|
