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| Status |
Public on Jun 30, 2014 |
| Title |
Ssn6, a new regulator of white-opaque switching in Candida albicans |
| Organism |
Candida albicans |
| Experiment type |
Genome binding/occupancy profiling by genome tiling array
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| Summary |
The human fungal pathogen Candida albicans can switch between two phenotypic cell types, termed “white” and “opaque.” Both cell types are heritable for many generations, and the switch between the two types occurs epigenetically, that is, without a change in the DNA sequence of the genome. In this work we describe that SSN6, the C. albicans functional homolog of Saccharomyces cerevisiae Cyc8, is a regulator of the white-opaque switch.
Chromatin IP's were performed using the protocol described by Hernday et al (Methods Enzymol. 2010;470:737-58. ). Briefly, log phase cultures were crosslinked with formaldehyde prior to cell lysis, chromatin shearing, and transcription-factor immunoprecipitation. Recovered DNA was amplified, dye-coupled, and competitively hybridized to a 244k-probe tiling array with a non-enriched genomic DNA reference.
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| Overall design |
Immunoprecipitated chromatin was hybridized against a non-enriched genomic DNA reference to identify transcription-factor binding sites
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| Contributor(s) |
Hernday A |
| Citation(s) |
26814177 |
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| Submission date |
May 28, 2014 |
| Last update date |
Feb 01, 2016 |
| Contact name |
Aaron Hernday |
| Organization name |
UC San Francisco
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| Street address |
600 15th st
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| City |
San Francisco |
| State/province |
Ca |
| ZIP/Postal code |
94156 |
| Country |
USA |
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| Platforms (1) |
| GPL16365 |
Candida albicans tiling ChIP array |
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| Samples (9)
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| GSM1400180 |
Ssn6-1004_AHY136a1_Array178_Normalized Log2 Ratio |
| GSM1400181 |
Ssn6-1004_AHY347a2_Array180_Normalized Log2 Ratio |
| GSM1400182 |
Ssn6-664_AHY135_Array226_Normalized Log2 Ratio |
| GSM1400183 |
Ssn6-664_AHY135a1_Array175_Normalized Log2 Ratio |
| GSM1400184 |
Ssn6-664_AHY136a1_Array177_Normalized Log2 Ratio |
| GSM1400185 |
Ssn6-664_AHY343_Array227_Normalized Log2 Ratio |
| GSM1400186 |
Ssn6-664_AHY347a2_Array179_Normalized Log2 Ratio |
| GSM1400187 |
Ssn6_Nmyc_AHY498_Array123 Normalized Log2 Ratio |
| GSM1400188 |
Untagged-myc_Opaque_Array136_Normalized Log2 Ratio |
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| Relations |
| BioProject |
PRJNA248763 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE58054_RAW.tar |
225.9 Mb |
(http)(custom) |
TAR (of GPR) |
| Processed data included within Sample table |
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