|
Status |
Public on Jun 30, 2014 |
Title |
Untagged-myc_Opaque_Array136_Normalized Log2 Ratio |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
AHY136_Opaque
|
Organism |
Candida albicans |
Characteristics |
chip antibody: myc phenotype: opaque
|
Treatment protocol |
formaldehyde crosslinking
|
Growth protocol |
25degC_SD+AA+uri
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Crosslinked cells were lysed by mechanical disruption. Genomic DNA was sheared by sonication then immunoprecipitated with monoclonal or polyclonal antibodies.
|
Label |
Cy5
|
Label protocol |
Aminoallyl dUTP was incorporated during linear amplification of the genomic DNA using exo- Klenow fragment
|
|
|
Channel 2 |
Source name |
AHY136_Opaque
|
Organism |
Candida albicans |
Characteristics |
chip antibody: No antibody phenotype: opaque
|
Treatment protocol |
formaldehyde crosslinking
|
Growth protocol |
25degC_SD+AA+uri
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Crosslinked cells were lysed by mechanical disruption. Genomic DNA was sheared by sonication then immunoprecipitated with monoclonal or polyclonal antibodies.
|
Label |
Cy3
|
Label protocol |
Aminoallyl dUTP was incorporated during linear amplification of the genomic DNA using exo- Klenow fragment
|
|
|
|
Hybridization protocol |
Hybridization followed the Agilent protocol
|
Scan protocol |
Arrays were scanned with an Axon 4000B scanner
|
Data processing |
Data was extracted and lowess normalized using Agilent ChIP analytics v1.3 software.
|
|
|
Submission date |
May 28, 2014 |
Last update date |
Jun 30, 2014 |
Contact name |
Aaron Hernday |
Organization name |
UC San Francisco
|
Street address |
600 15th st
|
City |
San Francisco |
State/province |
Ca |
ZIP/Postal code |
94156 |
Country |
USA |
|
|
Platform ID |
GPL16365 |
Series (1) |
GSE58054 |
Ssn6, a new regulator of white-opaque switching in Candida albicans |
|