|
| Status |
Public on Jun 30, 2014 |
| Title |
Untagged-myc_Opaque_Array136_Normalized Log2 Ratio |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
AHY136_Opaque
|
| Organism |
Candida albicans |
| Characteristics |
chip antibody: myc
phenotype: opaque
|
| Treatment protocol |
formaldehyde crosslinking
|
| Growth protocol |
25degC_SD+AA+uri
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Crosslinked cells were lysed by mechanical disruption. Genomic DNA was sheared by sonication then immunoprecipitated with monoclonal or polyclonal antibodies.
|
| Label |
Cy5
|
| Label protocol |
Aminoallyl dUTP was incorporated during linear amplification of the genomic DNA using exo- Klenow fragment
|
| |
|
| Channel 2 |
| Source name |
AHY136_Opaque
|
| Organism |
Candida albicans |
| Characteristics |
chip antibody: No antibody
phenotype: opaque
|
| Treatment protocol |
formaldehyde crosslinking
|
| Growth protocol |
25degC_SD+AA+uri
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Crosslinked cells were lysed by mechanical disruption. Genomic DNA was sheared by sonication then immunoprecipitated with monoclonal or polyclonal antibodies.
|
| Label |
Cy3
|
| Label protocol |
Aminoallyl dUTP was incorporated during linear amplification of the genomic DNA using exo- Klenow fragment
|
| |
|
| |
| Hybridization protocol |
Hybridization followed the Agilent protocol
|
| Scan protocol |
Arrays were scanned with an Axon 4000B scanner
|
| Data processing |
Data was extracted and lowess normalized using Agilent ChIP analytics v1.3 software.
|
| |
|
| Submission date |
May 28, 2014 |
| Last update date |
Jun 30, 2014 |
| Contact name |
Aaron Hernday |
| Organization name |
UC San Francisco
|
| Street address |
600 15th st
|
| City |
San Francisco |
| State/province |
Ca |
| ZIP/Postal code |
94156 |
| Country |
USA |
| |
|
| Platform ID |
GPL16365 |
| Series (1) |
| GSE58054 |
Ssn6, a new regulator of white-opaque switching in Candida albicans |
|
