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| Status |
Public on Jun 10, 2014 |
| Title |
Direct entry by RNase E is a major pathway for the degradation and processing of RNA in Escherichia coli |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Here we show using RNA-seq that cleavage by RNase E direct entry pervades in both the degradation and processing of RNA. We also give further evidence that direct entry is facilitated by cooperative interaction with segments in addition to the ones in which cleavage occurs.
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| Overall design |
RNA-seq profiles were compared between a temperature-sensitive mutant of rne and its congenic wild-type incubated at a non-permissive temperature. RNA seq profiles were also compared between samples before and after incubation with a 5'-sensing mutant of RNase E in vitro.
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| |
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| Contributor(s) |
Clarke JE, Kime L, Romero A D, McDowall KJ |
| Citation(s) |
25237058 |
| |
| Submission date |
Jun 06, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Kenneth McDowall |
| E-mail(s) |
K.J.McDowall@leeds.ac.uk
|
| Organization name |
University of Leeds
|
| Department |
Faculty of biological sciences
|
| Lab |
McDowall
|
| Street address |
Clarendon way
|
| City |
Leeds |
| ZIP/Postal code |
LS2 9JT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL14548 |
Illumina HiSeq 2000 (Escherichia coli) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA251996 |
| SRA |
SRP043044 |
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