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| Status |
Public on Jan 14, 2016 |
| Title |
Genome-wide hydroxymethylcytosine pattern changes in response to oxidative stress |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
The TET-family enzymes (TETs) convert methylcytosine to hydroxymethylcytosine, a lately discovered epigenetic modification that can modulate transcription. While recent reports suggest that TETs may play a role in response to oxidative stress, this role remains uncertain. Here we show that Tet1 is sensitive to peroxide and report a global decrease in hydroxymethylcytosine in cells treated with BSO and in the intestinal epithelium of mice lacking the major antioxidant enzymes glutathione peroxidases 1 and 2. Furthermore, genome-wide profiling revealed differentially hydroxymethylated regions in genes involved in responses to oxidative stress. Intriguingly, a considerable proportion of these regions lie in genes encoding microRNAs predicted to target transcripts involved in oxidative stress response. This work thus demonstrates a profound effect of oxidative stress on the hydroxymethylome and opens exciting new avenues of research by highlighting a set of microRNAs that may participate in the prevention or etiology of oxidative-stress-related diseases.
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| Overall design |
Examination of DNA hydroxymethylation landscape in SY5Y cell lines and in intestinal epithelium of mice.
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| Contributor(s) |
Delatte B, Jeschke J, Defrance M, Creppe C, Calonne E, Bizet M, Deplus R, Marroquí L, Libin M, Mascart F, Eizirik DL, Jeltsch A, Jurkowski TP, Fuks F |
| Citation missing |
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| |
| Submission date |
Jul 24, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Matthieu Defrance |
| Organization name |
Université Libre de Bruxelles
|
| Lab |
Cancer Epigenetics
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| Street address |
CP614 route de Lennik 808
|
| City |
Bruxelles |
| ZIP/Postal code |
1070 |
| Country |
Belgium |
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| Platforms (2) |
| GPL15456 |
Illumina HiScanSQ (Homo sapiens) |
| GPL16173 |
Illumina HiScanSQ (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA256032 |
| SRA |
SRP044774 |
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