NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE60236 Query DataSets for GSE60236
Status Public on Aug 28, 2014
Title Variation in primary CD4+ T cell response to activation across time and people
Organism Homo sapiens
Experiment type Expression profiling by array
Other
Summary This SuperSeries is composed of the SubSeries listed below.

Variation in individuals' adaptive immune response is believed to influence susceptibility to complex diseases in humans. The genetic basis of such variation is poorly understood. We analyzed the response of human CD4+ T cells from a cohort of 348 healthy humans, representing three major ancestries, to in vitro stimulation, alone or in Th17 conditions, or with IFNb stimulation.
 
Overall design The SuperSeries includes three SubSeries. The first is a pilot microarray time-course analysis (45' to 72 hrs) of CD4+ T cells from a single pool of donors, performed to define the key timepoints of the response. The second is a microarray analysis of responses, in 5 defined conditions (unbiased stimulation at 4 and 48 hrs, Th17 conditions, IFNb supplementation) of CD4+ T cells from 15 healthy individuals. The third is a focused signature analysis (Nanostring) of responses of 236 transcripts (also in the same 5 conditions) in CD4+ T cells of 348 healthy donors.
 
Citation(s) 25214635
Submission date Aug 08, 2014
Last update date Jul 26, 2018
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (2)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
GPL18938 Nanostring nCounter ImmVar CD4+ T cell Custom CodeSet
Samples (2054)
GSM1468418 Unstimulated 0hr
GSM1468419 Activated 45min
GSM1468420 IFNb stimulated 45min
This SuperSeries is composed of the following SubSeries:
GSE60234 Time course expression measured by microarray of fresh CD4+ response to activation from healthy individuals
GSE60235 Expression data measured by microarray of CD4+ T cells from healthy individuals stimulated with anti-CD3/CD28
GSE60341 Expression data measured by custom Nanostring gene set of CD4+ T cells from healthy individuals stimulated with anti-CD3/CD28 with or without IFNb or Th17 polarizing cytokines
Relations
BioProject PRJNA257800

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60236_RAW.tar 455.7 Mb (http)(custom) TAR (of CEL, RCC)

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.