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Series GSE60341 Query DataSets for GSE60341
Status Public on Aug 28, 2014
Title Expression data measured by custom Nanostring gene set of CD4+ T cells from healthy individuals stimulated with anti-CD3/CD28 with or without IFNb or Th17 polarizing cytokines
Organism Homo sapiens
Experiment type Other
Summary Variation in individuals' adaptive immune response is believed to influence susceptibility to complex diseases in humans. The genetic basis of such variation is poorly understood.
We measured gene expression from resting and activated CD4+ T cells derived from the peripheral blood of 348 healthy individuals. We activated the primary T cells with anti-CD3/CD28 beads.
 
Overall design We collected peripheral blood from each human donor. We isolated peripheral blood mononuclear cells by Ficoll, and negatively selected for CD4+ T cells using RosettaSep. We then either left cells unstimulated or stimulated them with beads conjugated with anti-CD3 and anti-CD28 either without additional cytokines, or with IFNb, or with Th17 cocktail. Cells were harvested at 0hr, 4hr (anti-CD3/CD28 +/- IFNb) or 48hr (anti-CD3/CD28 +/- Th17) and lysed, and RNA was isolated to be profiled on NanoString.
 
Contributor(s) Ye C, Feng T, Regev A, Benoist C
Citation(s) 25214635
Submission date Aug 12, 2014
Last update date Oct 06, 2014
Contact name Liang Yang
Organization name Harvard Medical School
Department Pathology
Lab CBDM
Street address 77 Avenue Louis Pasteur
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL18938 Nanostring nCounter ImmVar CD4+ T cell Custom CodeSet
Samples (1950)
GSM1472082 20120811_NC18_NC18_01
GSM1472083 20120811_NC18_NC18_02
GSM1472084 20120811_NC18_NC18_03
This SubSeries is part of SuperSeries:
GSE60236 Variation in primary CD4+ T cell response to activation across time and people
Relations
BioProject PRJNA258060

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60341_RAW.tar 7.2 Mb (http)(custom) TAR (of RCC)
GSE60341_nanostring_processed_data.txt.gz 2.5 Mb (ftp)(http) TXT
Processed data are available on Series record

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