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| Status |
Public on Dec 01, 2014 |
| Title |
Functional Antagonism Between CELF and Mbnl Proteins in Cytoplasm and Nucleus [hearts] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The CUG-BP and ETR-3-like factor 1 (Celf1) RNA binding protein plays an important role in heart and muscle development, and is over-expressed in the disease myotonic dystrophy. Celf1 has known roles in regulation of RNA splicing, RNA stability, and protein translation. To identify transcriptome-wide targets of the Celf1 protein in heart, we performed RNA-Seq of polyA+ RNA from mice inducibly expressing Celf1 in the muscle.
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| Overall design |
Mice were engineered to express the reverse tetracycline trans-activator (rtTA2S-M2) from the rate myosin light chain 1/3 promoter/enhancer, and an N-terminal Flag-tagged version of the LYLQ isoform of human Celf1 from a tet-inducible promoter. Mice were fed doxycycline to induce Celf1 expression in muscle, and gastrocnemius muscles were harvested from 3 mice each at 12 hour, 24 hour, 72 hour, and 7 day time points. To account for potential doxycycline-dependent effects, control mice were fed doxycycline for 72 hours but these mice did not contain the tet-inducible Celf1 cassette. In total, 15 gastrocnemius samples were analyzed by RNA-Seq.
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| Contributor(s) |
Wang ET, Ward A, Treacy D, Wang TT, Housman D, Cooper T, Burge C |
| Citation(s) |
25883322 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| RC2 HG005624 |
Deep Sequencing Analysis of mRNA Isoform Expression Changes in Myotonic Dystrophy |
MASSACHUSETTS INSTITUTE OF TECHNOLOGY |
CHRISTOPHER B BURGE, Housman, Thomas A Cooper |
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| Submission date |
Sep 30, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Eric T Wang |
| Organization name |
University of Florida
|
| Street address |
2033 Mowry Rd
|
| City |
Gainesville |
| State/province |
FL |
| ZIP/Postal code |
32610 |
| Country |
USA |
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| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE61893 |
Functional Antagonism Between CELF and Mbnl Proteins in Cytoplasm and Nucleus |
|
| Relations |
| BioProject |
PRJNA262629 |
| SRA |
SRP048523 |
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