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| Status |
Public on Oct 17, 2014 |
| Title |
Transcriptome analysis of transgenic plants with ectopic expression (driven by 35S promoter) of At14a-Like1 (AFL1, At3g28270) YFP fusion protein. |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Transcriptome analysis was used to identify genes differentially expressed in transgenic plants expressing At14a-Like1 (AFL1, At3g28270)) YFP fusion protein compared to wild type. Transcriptome analysis was performed using seedlings grown under either control conditions or low water potential stress with the objective of identifying genes associated with the increased growth caused by AFL1 overexpression under stress. The analysis identified 172 genes up-regulated and 525 genes down regulated in 35S:AFL1-YFP relative to wild type in control condtions. In the stress treatment 398 genes were up regulated and 722 down regulated in 35S:AFL1-YFP plants relative to wild type. The criteria used to identify up and down regulated genes was fold change greater than 1.5 and corrected p value ≤0.05.
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| Overall design |
Wild type (Col-0) or transgenic Arabidopsis thaliana seedlings were germinated on agar plates and after seven days of growth, seedlings were transferred either to fresh plates of control media or to low water potential (-1.2 MPa) stress plates. Samples were collected 10 h after transfer.
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| Contributor(s) |
Paul V, Nagaraj KM |
| Citation(s) |
26240315 |
| |
| Submission date |
Oct 16, 2014 |
| Last update date |
Apr 19, 2017 |
| Contact name |
Verslues Edwin Paul |
| Organization name |
Academia Sinica
|
| Department |
Institute of Plant and Microbial Biology
|
| Lab |
R426
|
| Street address |
128 Sec. 2, Academia Rd, Nankang, Taipei 11529 Taiwan, R.O.C.
|
| City |
Taipei |
| ZIP/Postal code |
11529 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL9020 |
Agilent-021169 Arabidopsis 4 Oligo Microarray (V4) (Feature Number version) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA264051 |
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