|
| Status |
Public on Nov 17, 2006 |
| Title |
C. elegans small RNA sequences from wild type animals fed on sel-1 dsRNA producing bacteria |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
RNA interference (RNAi) is a phylogenetically widespread gene silencing process triggered by doublestranded RNA (dsRNA). In plants and C. elegans, two distinct populations of small RNAs have been proposed to participate in RNAi : "Primary siRNAs" (derived from Dicer nuclease-mediated cleavage of the original trigger) and "Secondary siRNAs" (additional small RNAs whose synthesis requires an RNA-directed RNA polymerase [RdRP]). Analyzing small RNAs associated with ongoing RNAi in C. elegans, we found secondary siRNAs to comprise the vast majority. The bulk of secondary siRNAs exhibited structure and sequence indicative of a biosynthetic mode where each molecule derives from an independent de novo initiation by RdRP. Analysis of endogenous small RNAs indicated that a fraction derive from a biosynthetic mechanism that is similar to that of secondary siRNAs formed during RNAi, suggesting that small antisense transcripts derived from cellular mRNAs by RdRP activity may have key roles in cellular regulation.
Keywords: C. elegans small RNA sequences from wild type animals fed on sel-1 dsRNA producing bacteria
|
| |
|
| Overall design |
C. elegans small RNA sequences from wild type animals fed on sel-1 dsRNA producing bacteria
|
| |
|
| Contributor(s) |
Pak J, Fire A |
| Citation(s) |
17124291 |
| |
| Submission date |
Nov 15, 2006 |
| Last update date |
Mar 16, 2012 |
| Contact name |
Andrew Fire |
| E-mail(s) |
afire@stanford.edu
|
| Phone |
650-723-2885
|
| Fax |
650-724-9070
|
| Organization name |
Stanford University
|
| Department |
Pathology and Genetics
|
| Street address |
300 Pasteur Drive RML235 M5324
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305-5324 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL4569 |
C. elegans small RNA sequences |
|
| Samples (1) |
| GSM146128 |
Caenorhabditis elegans small RNA sequences from wild type animals fed on sel-1 dsRNA producing bacteria |
|
| Relations |
| BioProject |
PRJNA99679 |
