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| Status |
Public on Jul 01, 2015 |
| Title |
Expression data from embryonic chicken feather and scale skins |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by array
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| Summary |
Epithelial appendages are the product of epithelial – mesenchymal interactions. Tissue recombination experiments showed that in general, the dermis determines the phenotype of the epithelial appendage. Chicken dorsal skin epithelium interacts with its underlying mesenchyme to form feathers beginning at E7 (H&H stage 31), while metatarsal scale epithelium interacts with its mesenchyme to form scales beginning at E9 (H&H stage 35) which stabilize around E12 (H&H stage 38).
We sought to evaluate the molecular differences of tissues with different competence and inductive abilities to form feathers and scales.
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| Overall design |
Chicken embryos were selected to obtain competent E7 and non-competent at E9 feather forming skin from dorsal. The competent E9 and non-competent E11 meta-tarsal scale forming skin from metatarsal were selected for examing the differences in regional specificity. Epithelium and mesenchyme from each skin were prepared separately. Samples were prepared for RNA extraction and hybridization on Affymetrix microarrays. We gathered 8 sets of samples for the analysis: undifferentiated E7 feather skin epithelium (E7fe) and mesenchyme (E7fm); differentiated E9 feather skin epithelium (E9fe) and mesenchyme (E9fm); undifferentiated E9 scale skin epithelium (E9se) and mesenchyme (E9sm); and differentiated E11 scale skin epithelium (E11se) and mesenchyme (E11sm)
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| Contributor(s) |
Hughes MW, Chuong C |
| Citation(s) |
25612663 |
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| Submission date |
Oct 31, 2014 |
| Last update date |
Oct 23, 2015 |
| Contact name |
Shau-Ping Lin |
| E-mail(s) |
shaupinglin@ntu.edu.tw
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| Organization name |
National Taiwan University
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| Department |
Institute of Biotechnology
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| Street address |
No. 81, Chang-Xing St.
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| City |
Taipei City |
| ZIP/Postal code |
10672 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL3213 |
[Chicken] Affymetrix Chicken Genome Array |
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| Samples (20)
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| GSM1535605 |
E7 feather mesenchyme, biological rep2 |
| GSM1535606 |
E9 feather epithelium, biological rep1 |
| GSM1535607 |
E9 feather epithelium, biological rep2 |
| GSM1535608 |
E9 feather epithelium, biological rep3 |
| GSM1535609 |
E9 feather mesenchyme, biological rep1 |
| GSM1535610 |
E9 feather mesenchyme, biological rep2 |
| GSM1535611 |
E9 feather mesenchyme, biological rep3 |
| GSM1535612 |
E9 scale epithelium, biological rep1 |
| GSM1535613 |
E9 scale epithelium, biological rep2 |
| GSM1535614 |
E9 scale mesenchyme, biological rep1 |
| GSM1535615 |
E9 scale mesenchyme, biological rep2 |
| GSM1535616 |
E11 scale epithelium, biological rep1 |
| GSM1535617 |
E11 scale epithelium, biological rep2 |
| GSM1535618 |
E11 scale epithelium, biological rep3 |
| GSM1535619 |
E11 scale mesenchyme, biological rep1 |
| GSM1535620 |
E11 scale mesenchyme, biological rep2 |
| GSM1535621 |
E11 scale mesenchyme, biological rep3 |
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| Relations |
| BioProject |
PRJNA265926 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE62882_RAW.tar |
66.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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