|
| Status |
Public on Jul 01, 2015 |
| Title |
E7 feather epithelium, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Chicken embryonic dorsal feather skin epithelium, undifferentiated, pooled
|
| Organism |
Gallus gallus |
| Characteristics |
tissue: embryonic skin
breed: white leghorn
age: Embryonic Day 7 (E7)
|
| Treatment protocol |
Chick skins were dissected in HBSS. Skins were then treated with 2X CMF in 0.25% EDTA, pH 7.5 for 10 min on ice to separate the epidermis and dermis.
|
| Growth protocol |
Fertilized egg incubated at 39oC, 50~60% humidity
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45oC on Affymetrix Chicken Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
Gene expression data from undifferentiated dorsal feather skin epithelium
|
| Data processing |
The data were read, analyzed, and normalized with RMA in the affy package in default setting under R statististical computing software environment.
|
| |
|
| Submission date |
Oct 31, 2014 |
| Last update date |
Jul 01, 2015 |
| Contact name |
Shau-Ping Lin |
| E-mail(s) |
shaupinglin@ntu.edu.tw
|
| Organization name |
National Taiwan University
|
| Department |
Institute of Biotechnology
|
| Street address |
No. 81, Chang-Xing St.
|
| City |
Taipei City |
| ZIP/Postal code |
10672 |
| Country |
Taiwan |
| |
|
| Platform ID |
GPL3213 |
| Series (1) |
| GSE62882 |
Expression data from embryonic chicken feather and scale skins |
|
