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| Status |
Public on Mar 31, 2015 |
| Title |
Control of the E. coli SOS regulon expression by intracellular dNTP pool changes |
| Platform organisms |
Escherichia coli O157:H7 str. EDL933; Escherichia coli CFT073; Escherichia coli O157:H7 str. Sakai; Escherichia coli str. K-12 substr. MG1655 |
| Sample organism |
Escherichia coli |
| Experiment type |
Expression profiling by array
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| Summary |
We studied SOS mutator effect mediated by recA730 and changes in the dNTP pool. We found that established dNTP pool changes resulting from deficiencies in the ndk or dcd genes, had a strongly suppressive effect on the recA730 mutator effect. To investigate whether the observed reduction in SOS mutator effect is due to lowered expression of the entire SOS regulon, we performed microarray analysis of gene expression profiles in each of the single ndk, dcd, and recA730 strains, as well as the double recA730 ndk and recA730 dcd strains.
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| Overall design |
Comparison of transcriptomes of the bacterium Escherichia coli six strains: wt, dcd, ndk, recA730, recA730 dcd and recA730 ndk. All strains were derivatives of the MC4100 strain, carrying a sulA366 allele (Δ(argF-lac)169 sulA366). dcd and ndk alleles used were dcd::kan and ndk::cam, respectively. Strains were compared in pairs: wt vs dcd, wt vs ndk, wt vs recA730, recA730 vs recA730 dcd and recA730 vs recA730 ndk. Two or three biological replicates for each strain were used. Each biological replicate had two technical replicates with dye swapping.
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| Contributor(s) |
Masłowska K, Makiela-Dzbenska K, Fijałkowska I, Schaaper R |
| Citation(s) |
25824947 |
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| Submission date |
Oct 31, 2014 |
| Last update date |
Apr 07, 2015 |
| Contact name |
Iwona Fijalkowska |
| E-mail(s) |
iwonaf@ibb.waw.pl
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| Organization name |
Institute of Biochemistry and Biophysics
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| Street address |
Pawinskiego 5A
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| City |
Warszawa |
| ZIP/Postal code |
02-106 |
| Country |
Poland |
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| Platforms (1) |
| GPL13359 |
Agilent-020097 E. coli Gene Expression Microarray (Probe Name version) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA265996 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE62898_RAW.tar |
45.6 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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