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Status |
Public on Nov 10, 2014 |
Title |
RNA bisulfite sequencing of 25S rRNA (C2278) in S. cerevisiae and of 28S rRNA (C3381) in C. elegans |
Organisms |
Saccharomyces cerevisiae; Caenorhabditis elegans |
Experiment type |
Other
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Summary |
The RNA methyltransferase NSUN5 modulates the lifespans of yeast, worms and flies by re-programming the translation of specific subsets of mRNAs, which are associated with the cellular stress response. Here we show by RNA bisulfite sequencing that Rcm1 specifically methylates C2278 of yeast 25S rRNA and nsun-5 C3381 of C. elegans 28S rRNA.
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Overall design |
In S. cerevisiae RNA was isolated from polysome and monosome fractions of two genotypes (wild-type and rcm1 deletion) and three stress treatments (unstressed, 30 min and 90 min H2O2) in single replicates. In C. elegans total RNA of wild-type (N2) and JGG1 (nsun-5 mutant) were compared in single replicates.
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Contributor(s) |
Schosserer M, Grillari J, Breitenbach-Koller L, Gerstl MP, Weinhaeusel A, Sonntagbauer M |
Citation(s) |
25635753 |
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Submission date |
Nov 07, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Markus Schosserer |
E-mail(s) |
markus.schosserer@meduniwien.ac.at
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Organization name |
Medical University of Vienna
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Department |
Center for Pathobiochemistry and Genetics
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Lab |
Markus Schosserer
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Street address |
Waehringer Strasse 10
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City |
Vienna |
ZIP/Postal code |
1090 |
Country |
Austria |
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Platforms (2) |
GPL16028 |
Ion Torrent PGM (Saccharomyces cerevisiae) |
GPL19396 |
Ion Torrent PGM (Caenorhabditis elegans) |
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Samples (14)
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GSM1541512 |
yeast wild-type, unstressed, polysomes |
GSM1541513 |
yeast wild-type, unstressed, monosomes |
GSM1541514 |
yeast wild-type, 30 min H2O2, polysomes |
GSM1541515 |
yeast wild-type, 30 min H2O2, monosomes |
GSM1541516 |
yeast wild-type, 90 min H2O2, polysomes |
GSM1541517 |
yeast wild-type, 90 min H2O2, monosomes |
GSM1541518 |
yeast rcm1 knockout, unstressed, polysomes |
GSM1541519 |
yeast rcm1 knockout, unstressed, monosomes |
GSM1541520 |
yeast rcm1 knockout, 30 min H2O2, polysomes |
GSM1541521 |
yeast rcm1 knockout, 30 min H2O2, monosomes |
GSM1541522 |
yeast rcm1 knockout, 90 min H2O2, polysomes |
GSM1541523 |
yeast rcm1 knockout, 90 min H2O2, monosomes |
GSM1541524 |
C. elegans N2 wild-type |
GSM1541525 |
C. elegans JGG1 nsun-5 mutant |
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Relations |
BioProject |
PRJNA266730 |
SRA |
SRP049646 |
Supplementary file |
Size |
Download |
File type/resource |
GSE63113_RAW.tar |
30.0 Kb |
(http)(custom) |
TAR (of CSV) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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