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Series GSE63113 Query DataSets for GSE63113
Status Public on Nov 10, 2014
Title RNA bisulfite sequencing of 25S rRNA (C2278) in S. cerevisiae and of 28S rRNA (C3381) in C. elegans
Organisms Saccharomyces cerevisiae; Caenorhabditis elegans
Experiment type Other
Summary The RNA methyltransferase NSUN5 modulates the lifespans of yeast, worms and flies by re-programming the translation of specific subsets of mRNAs, which are associated with the cellular stress response. Here we show by RNA bisulfite sequencing that Rcm1 specifically methylates C2278 of yeast 25S rRNA and nsun-5 C3381 of C. elegans 28S rRNA.
 
Overall design In S. cerevisiae RNA was isolated from polysome and monosome fractions of two genotypes (wild-type and rcm1 deletion) and three stress treatments (unstressed, 30 min and 90 min H2O2) in single replicates. In C. elegans total RNA of wild-type (N2) and JGG1 (nsun-5 mutant) were compared in single replicates.
 
Contributor(s) Schosserer M, Grillari J, Breitenbach-Koller L, Gerstl MP, Weinhaeusel A, Sonntagbauer M
Citation(s) 25635753
Submission date Nov 07, 2014
Last update date May 15, 2019
Contact name Markus Schosserer
E-mail(s) markus.schosserer@meduniwien.ac.at
Organization name Medical University of Vienna
Department Center for Pathobiochemistry and Genetics
Lab Markus Schosserer
Street address Waehringer Strasse 10
City Vienna
ZIP/Postal code 1090
Country Austria
 
Platforms (2)
GPL16028 Ion Torrent PGM (Saccharomyces cerevisiae)
GPL19396 Ion Torrent PGM (Caenorhabditis elegans)
Samples (14)
GSM1541512 yeast wild-type, unstressed, polysomes
GSM1541513 yeast wild-type, unstressed, monosomes
GSM1541514 yeast wild-type, 30 min H2O2, polysomes
Relations
BioProject PRJNA266730
SRA SRP049646

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE63113_RAW.tar 30.0 Kb (http)(custom) TAR (of CSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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