|
| Status |
Public on Dec 12, 2014 |
| Title |
Heterochromatin assembly and transcriptome repression by Set1 in coordination with a class II histone deacetylase |
| Organism |
Schizosaccharomyces pombe |
| Experiment type |
Expression profiling by array
Genome binding/occupancy profiling by array
|
| Summary |
Gene expression profiling of S. pombe set1/COMPASS/H3K4 mutants, atf1 and set1 atf1 deletion mutants, set1 clr3 deletion mutants; ChIP-chip tiling microarray profiling of S. pombe Set1, Atf1, H3K4me3 in wt/atf1 deletion mutants, H3K9me2 in wt/set1 clr3 deletion mutants
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| |
|
| Overall design |
Custom Agilent 60mer microarrays were used to assay gene expression in set1/COMPASS mutants in combination with atf1 and clr3 mutants, and to profile genome-wide binding of Set1, Atf1, H3K4me3 and H3K9me2 in S. pombe cells (two-color mutant vs. wildtype, ChIP vs. input experiments).
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| |
|
| Contributor(s) |
Cam HP, Lorenz DR |
| Citation(s) |
25497836 |
| |
| Submission date |
Nov 14, 2014 |
| Last update date |
Sep 19, 2016 |
| Contact name |
Hugh P Cam |
| E-mail(s) |
hugh.cam@bc.edu
|
| Phone |
6175526851
|
| Organization name |
Boston College
|
| Department |
Biology
|
| Street address |
Higgins Hall Room 355, 140 Commonwealth Avenue
|
| City |
Chestnut Hill |
| State/province |
MA |
| ZIP/Postal code |
02467 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6503 |
Agilent-016010 S.pombe 4X44K_v3 (Feature Number version) |
|
| Samples (40)
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|
| Relations |
| BioProject |
PRJNA267234 |
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