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Status |
Public on Dec 12, 2014 |
Title |
Heterochromatin assembly and transcriptome repression by Set1 in coordination with a class II histone deacetylase |
Organism |
Schizosaccharomyces pombe |
Experiment type |
Expression profiling by array Genome binding/occupancy profiling by array
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Summary |
Gene expression profiling of S. pombe set1/COMPASS/H3K4 mutants, atf1 and set1 atf1 deletion mutants, set1 clr3 deletion mutants; ChIP-chip tiling microarray profiling of S. pombe Set1, Atf1, H3K4me3 in wt/atf1 deletion mutants, H3K9me2 in wt/set1 clr3 deletion mutants
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Overall design |
Custom Agilent 60mer microarrays were used to assay gene expression in set1/COMPASS mutants in combination with atf1 and clr3 mutants, and to profile genome-wide binding of Set1, Atf1, H3K4me3 and H3K9me2 in S. pombe cells (two-color mutant vs. wildtype, ChIP vs. input experiments).
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Contributor(s) |
Cam HP, Lorenz DR |
Citation(s) |
25497836 |
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Submission date |
Nov 14, 2014 |
Last update date |
Sep 19, 2016 |
Contact name |
Hugh P Cam |
E-mail(s) |
hugh.cam@bc.edu
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Phone |
6175526851
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Organization name |
Boston College
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Department |
Biology
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Street address |
Higgins Hall Room 355, 140 Commonwealth Avenue
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City |
Chestnut Hill |
State/province |
MA |
ZIP/Postal code |
02467 |
Country |
USA |
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Platforms (1) |
GPL6503 |
Agilent-016010 S.pombe 4X44K_v3 (Feature Number version) |
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Samples (40)
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Relations |
BioProject |
PRJNA267234 |