|
Status |
Public on Jan 20, 2007 |
Title |
E2F4 and Myc ChIP-chip perfomed on Nimblegen hg17 ENCODE arrays |
Project |
Pilot ENCODE
|
Organism |
Homo sapiens |
Experiment type |
Genome binding/occupancy profiling by genome tiling array
|
Summary |
Identifying the chromosomal targets of transcription factors is important for reconstructing the transcriptional regulatory networks underlying global gene expression programs. ChIP-chip is a powerful technique to map chromosomal targets of transcription factors. We applied ChIP-chip to map downstream targets of the transcription factor E2F4 and c-Myc. E2F4 is a member of the E2F family of transcriptional regulators that functions as a repressor in quiescent and early G1 cells. c-Myc is a oncogenic transcription factor implicated in almost all aspects of tumorigenesis. We mapped E2F4 and c-Myc binding sites in 1% of the human genome using Nimblegen hg17 ENCODE arrays. Keywords: ChIP-chip
|
|
|
Overall design |
Cultured cells are cross-linked in vivo using 1% formaldehyde and chromatin is sheared to less than 1 kb fragments using a sonicator. Specific protein-dna complexes are pulled down using a specific antibody. After adequate washing, the protein-dna complexes are eluted, reverse cross-linked and the DNA purified by phenol-chloroform extraction. ChIP-enriched DNA is now labeled with Cy5 while DNA derived from a mock IP is used as a reference and labeled with Cy3. Samples are then hybridized on ENCODE arrays.
|
Web link |
http://www.ncbi.nlm.nih.gov/projects/geo/info/ENCODE.html
|
|
|
Contributor(s) |
Kim J, Bhinge A, Iyer V, Singer M, Jiang N, Green R |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
BioProject |
PRJNA63421 |
|
Submission date |
Nov 27, 2006 |
Last update date |
Jun 28, 2012 |
Contact name |
Vishy Iyer |
E-mail(s) |
iyerlab@gmail.com
|
Phone |
5122327833
|
Organization name |
University of Texas at Austin
|
Department |
Molecular Biosciences
|
Street address |
2500 Speedway Dr. MBB 3.212
|
City |
Austin |
State/province |
TX |
ZIP/Postal code |
78712 |
Country |
USA |
|
|
Platforms (1) |
GPL3514 |
ENCODE NimbleGen hg17_04252005 tiling array |
|
Samples (12)
|
GSM147141 |
E2F4 chip-chip in serum starved 2091 fibroblasts replicate 1 |
GSM147142 |
E2F4 chip-chip in serum starved 2091 fibroblasts replicate 2 |
GSM147143 |
E2F4 chip-chip in serum starved 2091 fibroblasts replicate 3 |
GSM147292 |
Myc ChIP-chip in serum starved fibroblasts set 1 |
GSM147322 |
Myc ChIP-chip in serum starved fibroblasts set 2 |
GSM147323 |
Myc ChIP-chip in serum starved fibroblasts set 3 |
GSM147324 |
Myc ChIP-chip in serum stimulated fibroblasts set 1 |
GSM147325 |
Myc ChIP-chip in serum stimulated fibroblasts set 2 |
GSM147326 |
Myc ChIP-chip in serum stimulated fibroblasts set 3 |
GSM147327 |
Myc ChIP-chip in HeLa cells replicate 1 |
GSM147328 |
Myc ChIP-chip in HeLa cells replicate 2 |
GSM147329 |
Myc ChIP-chip in HeLa cells replicate 3 |
|