|
| Status |
Public on Feb 04, 2015 |
| Title |
Differential gene regulation by the disease-associated short isoform of GATA1 (microarray) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
The transcriptional activiy of GATA1s was compared to GATA1 through gene expression analysis in a cell line model with both erythroid and megakaryocyte differentiation.
|
| |
|
| Overall design |
G1ME cells were derived from Gata1- mouse ES cells and have both megakaryocyte and erythrocyte differentiation potential upon reconstitution of GATA-1 expression (Stachura 2006). HA-tagged full length or short GATA-1 were expressed in G1ME cells grown in TPO via retroviral transductions. The cells were sorted for GFP positivity 68 hours post-transduction and then were allowed to recover in normal growth medium for 4h. Total RNA was then isolated using RNeasy kit from Qiagen 72 hours post-transduction.
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| |
|
| Contributor(s) |
Chlon T, Crispino J |
| Citation(s) |
25682601 |
| |
| Submission date |
Dec 23, 2014 |
| Last update date |
Jan 16, 2019 |
| Contact name |
Timothy Michael Chlon |
| E-mail(s) |
tchlon@u.northwestern.edu, timothy.chlon@cchmc.org, tchlon@gmail.com
|
| Organization name |
Cincinnati Children's
|
| Department |
Oncology
|
| Lab |
Wells
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6887 |
Illumina MouseWG-6 v2.0 expression beadchip |
|
| Samples (9)
|
|
| This SubSeries is part of SuperSeries: |
| GSE64496 |
Global transcriptome and chromatin occupancy analysis reveal the short isoform of GATA1 is deficient for erythroid specification and gene expression |
|
| Relations |
| BioProject |
PRJNA271111 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE64494_5993312065.sdf.gz |
3.3 Kb |
(ftp)(http) |
SDF |
| GSE64494_5993312065_fiducial__1_Red_center.tif.gz |
230.7 Kb |
(ftp)(http) |
TIFF |
| GSE64494_5993312065_fiducial__2_Red_center.tif.gz |
231.1 Kb |
(ftp)(http) |
TIFF |
| GSE64494_5993312065_fiducial__3_Red_center.tif.gz |
230.1 Kb |
(ftp)(http) |
TIFF |
| GSE64494_5993312085.sdf.gz |
3.3 Kb |
(ftp)(http) |
SDF |
| GSE64494_5993312085_fiducial__1_Red_center.tif.gz |
231.9 Kb |
(ftp)(http) |
TIFF |
| GSE64494_5993312085_fiducial__2_Red_center.tif.gz |
232.3 Kb |
(ftp)(http) |
TIFF |
| GSE64494_5993312085_fiducial__3_Red_center.tif.gz |
228.5 Kb |
(ftp)(http) |
TIFF |
| GSE64494_RAW.tar |
421.4 Mb |
(http)(custom) |
TAR (of IDAT, LOCS, TIFF, TXT, XML) |
| GSE64494_non-normalized.txt.gz |
1.7 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
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