|
| Status |
Public on Feb 04, 2015 |
| Title |
GATA1s A |
| Sample type |
RNA |
| |
|
| Source name |
G1ME
|
| Organism |
Mus musculus |
| Characteristics |
cell line: G1ME
genotype: Mutant: derived from Gata1-null ES cells
cell type: GATA1-deficient cell line with megakaryocyte and erythrocyte differentiation potential
media: Cells grown in TPO supernatant (10% v/v)
retrovirus: MigR1-HA-GATA1s
cell type: Sorted GFP-postive cells
|
| Treatment protocol |
Retroviral supernatant was added to the cells at 1e6 cells per ml, and the culture was centrifuged at 3200rpm for 90min. The cells were incuabted in the virus for an additional 4h, and then the virus was removed. The cells were crosslinked 48h later.
|
| Growth protocol |
G1ME cells were cultured in 1% TPO supernatant (v/v)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy column (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Standard Illumina protocol
|
| |
|
| Hybridization protocol |
Standard illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
Replicate 1
SAMPLE 3
|
| Data processing |
Data were quantile normalized and background was subtracted with IlluminaGUI
|
| |
|
| Submission date |
Dec 23, 2014 |
| Last update date |
Feb 05, 2015 |
| Contact name |
Timothy Michael Chlon |
| E-mail(s) |
tchlon@u.northwestern.edu, timothy.chlon@cchmc.org, tchlon@gmail.com
|
| Organization name |
Cincinnati Children's
|
| Department |
Oncology
|
| Lab |
Wells
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
OH |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platform ID |
GPL6887 |
| Series (2) |
| GSE64494 |
Differential gene regulation by the disease-associated short isoform of GATA1 (microarray) |
| GSE64496 |
Global transcriptome and chromatin occupancy analysis reveal the short isoform of GATA1 is deficient for erythroid specification and gene expression |
|
