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Series GSE64980 Query DataSets for GSE64980
Status Public on May 22, 2015
Title The fission yeast MTREC complex targets CUTs and unspliced mRNAs to the nuclear exosome [RIP-ChIP]
Organism Schizosaccharomyces pombe
Experiment type Expression profiling by array
Summary Cryptic unstable transcripts (CUTs) are rapidly degraded by the nuclear exosome, however, the way they are recognized and targeted to the exosome is not fully understood. The recently identified Schizosaccharomyces pombe MTREC complex has been shown to promote degradation of meiotic mRNAs and regulatory ncRNAs. Here, we report that the MTREC complex is also the major nuclear exosome targeting complex for CUTs and unspliced mRNAs. MTREC complex specifically binds to CUTs, meiotic mRNAs and unspliced mRNA transcripts and targets these RNAs for degradation by the nuclear exosome, while the TRAMP complex has only a minor role in this process. The MTREC complex physically interacts with the nuclear exosome and with various RNA-binding and -processing complexes, coupling RNA processing to the RNA degradation machinery. Our study reveals the central role of the evolutionarily conserved MTREC complex in RNA quality control, and in the recognition and elimination of aberrant, cryptic transcripts.
 
Overall design All experiments were performed twice in biological replicates.
 
Contributor(s) Zhou Y, Zhu J, Schermann G, Ohle C, Bendrin K, Sugioka-Sugiyama R, Sugiyama T, Fischer T
Citation(s) 25989903
Submission date Jan 14, 2015
Last update date Aug 24, 2015
Contact name Tamas Fischer
E-mail(s) tamas.fischer@bzh.uni-heidelberg.de
Phone +49 6221 544728
Organization name Biochemie Zentrum Heidelebrg
Department BZH
Lab AG Fischer
Street address Im Neuenheimer Feld 328
City Heidelberg
ZIP/Postal code 69120
Country Germany
 
Platforms (1)
GPL19654 Agilent-036227 Schizosaccharomyces pombe sp40c [ProbeName version]
Samples (6)
GSM1585479 Red1-Flag-TEV-protein A(FTP) -1
GSM1585480 Red1-Flag-TEV-protein A(FTP) -2
GSM1585481 Mtl1-Flag-TEV-protein A(FTP) -1
This SubSeries is part of SuperSeries:
GSE64992 The fission yeast MTREC complex targets CUTs and unspliced mRNAs to the nuclear exosome
Relations
BioProject PRJNA272675

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE64980_RAW.tar 101.3 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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