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Series GSE65366 Query DataSets for GSE65366
Status Public on Jan 29, 2015
Title Gene expression of human keratinocyte (HaCaT) under the stress of high sodium concentration
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The mechanisms by which the epidermis responds to disturbances in barrier function and restores homeostasis are unknown. With a disruption of the epidermal barrier, water is lost resulting in an increase in extracellular sodium concentration. We demonstrate that the sodium channel Nax functions as the sodium sensor. With increased extracellular sodium, Nax up-regulates prostasin which results in activation of the sodium channel ENaC, resulting in increased sodium flux and increased downstream mRNA synthesis of inflammatory mediators. The same pathways are present in lung epithelial cells.
A signal transduction pathway mediated directly through Nax and secondarily through ENaC results in production of secretory inflammatory mediators. These mediators result in epithelial proliferation and restoration of epidermal homeostasis, but can also have negative effects including excess inflammation and ultimately leads to activation of fibroblasts.
 
Overall design Both wild type and Nax knockdown HaCaT cells were seeded in 12-well plates. 70-80% confluent cells were starved with fetal bovine serum (FBS) free Dulbecco's modiļ¬ed Eagle's medium (DMEM) overnight followed by a treatment with control medium (final150 mM sodium) or medium plus 10% more sodium (final 165 mM sodium). Cells were harvested at time point 0, 4, and 16 hours post treatment.
 
Contributor(s) Xu W, Hong SJ, Zhong A, Jia S, Xie P, Leung KP, Galiano RD, Mustoe TA
Citation(s) 26537257
Submission date Jan 28, 2015
Last update date Oct 29, 2018
Contact name Wei Xu
E-mail(s) wei-xu@northwestern.edu
Phone 312 908 0566
Organization name Northwestern University, Feinberg School of Medicine
Department Surgery
Lab Laboratory for Wound Repair and Regenerative Medicine
Street address 303 E Chicago Ave, Tarry 4-714
City Chicago
State/province IL
ZIP/Postal code 60611
Country USA
 
Platforms (1)
GPL17586 [HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version]
Samples (30)
GSM1593835 HaCaT wild type control sodium concentration_1
GSM1593836 HaCaT wild type control sodium concentration_2
GSM1593837 HaCaT wild type control sodium concentration_3
Relations
BioProject PRJNA273807

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE65366_RAW.tar 767.9 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table
Processed data provided as supplementary file
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