|
| Status |
Public on Jan 29, 2015 |
| Title |
Nax knockdown HaCaT high sodium concentration 16 hour stimulation_2 |
| Sample type |
RNA |
| |
|
| Source name |
keratinocyte cell line
|
| Organism |
Homo sapiens |
| Characteristics |
gender: female
tissue: skin epithelium
cell line: HaCaT
genotype/variation: Nax knockdown
treatment: high sodium concentration
time: 16 hour
|
| Treatment protocol |
A treatment with control medium (final150 mM sodium) or medium plus 10% more sodium (final 165 mM sodium) was applied to each well. Cells were harvested at time point 0, 4, and 16 hours post treatment.
|
| Growth protocol |
Both wild type and Nax knockdown HaCaT cells were seeded in 12-well plates. 70-80% confluent cells were starved with fetal bovine serum (FBS) free Dulbecco's modiļ¬ed Eagle's medium (DMEM) overnight
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA from wild type and Nax knockdown HaCaT cells
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization performed at the Interdisciplinary Center for Biotechnology Research Microarray Core, University of Florida. The microarray chips were Affymetrix GeneChipĀ® Human Transcriptome Array 2.0
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
Sample name: HT_Nx_16h_2
|
| Data processing |
The microarray data was processed with Affymetrix Expression Console and Transcriptome Analysis Console (Affymetrix).
RMA expression value derived from Expression Console software; core-exon analysis
|
| |
|
| Submission date |
Jan 28, 2015 |
| Last update date |
Jan 29, 2015 |
| Contact name |
Wei Xu |
| E-mail(s) |
wei-xu@northwestern.edu
|
| Phone |
312 908 0566
|
| Organization name |
Northwestern University, Feinberg School of Medicine
|
| Department |
Surgery
|
| Lab |
Laboratory for Wound Repair and Regenerative Medicine
|
| Street address |
303 E Chicago Ave, Tarry 4-714
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60611 |
| Country |
USA |
| |
|
| Platform ID |
GPL17586 |
| Series (1) |
| GSE65366 |
Gene expression of human keratinocyte (HaCaT) under the stress of high sodium concentration |
|
