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| Status |
Public on Jul 01, 2015 |
| Title |
Molecular characterization of Notch1 positive progenitor cells in the developing retina |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The oscillatory expression of Notch signaling in neural progenitors suggests that both repressors and activators of neural fate specification are expressed in the same progenitors. Since Notch1 regulates photoreceptor differentiation and contributes (together with Notch3) to ganglion cell fate specification, we hypothesized that genes encoding photoreceptor and ganglion cell fate activators would be highly expressed in Notch1 receptor-bearing (Notch1+) progenitors, directing these cells to differentiate into photoreceptors or into ganglion cells when Notch1 activity is diminished.
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| Overall design |
To identify these genes, we used microarray analysis to study expression profiles of whole retinas and isolated from them Notch1+ cells at embryonic day 14 (E14) and postnatal day 0 (P0). To isolate Notch1+ cells, we utilized immunomagnetic cell separation. RNA from Notch1+ cells and whole retinas at E14 and P0 was extracted and used for microarray analysis. We processed individual samples that each contained 200,000-500,000 Notch1+ cells. A total of three independent biological replicates in the early stage (E14) of retinal development and four independent biological replicates in the late stage (P0) of retinal development were obtained for comparative profiling of Notch1+ cells and whole retinas.
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| Contributor(s) |
Ivanov D |
| Citation(s) |
26091508 |
| |
| Submission date |
Feb 17, 2015 |
| Last update date |
Sep 30, 2015 |
| Contact name |
Dmitry Ivanov |
| E-mail(s) |
divanov@med.miami.edu
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| Phone |
305-482-4230
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| Organization name |
University of Miami Miller School of Medicine
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| Department |
Ophthalmology
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| Street address |
1638 NW 10th Avenue
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| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33136 |
| Country |
USA |
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| Platforms (1) |
| GPL7656 |
Mouse Exonic Evidence Based Oligonucleotide (MEEBO) spotted microarrays |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA275624 |
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