|
| Status |
Public on Oct 08, 2015 |
| Title |
Disruption of histone methylation in developing sperm impairs offspring health transgenerationally [sperm] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
A father’s lifetime experiences can be transmitted to his offspring to affect
health and development. The mechanisms underlying paternal epigenetic
transmission are unclear. Unlike somatic cells, there are few nucleosomes
in sperm and their function in epigenetic inheritance is unknown. We
generated transgenic mice in which overexpression of the histone H3 lysine
4 (H3K4) demethylase LSD1/KDM1A during spermatogenesis reduced
H3K4 dimethylation in sperm. KDM1A overexpression in one generation
severely impaired development and survivability of offspring. These
defects persisted transgenerationally in the absence of KDM1A germ line
expression and were associated with altered RNA profiles in sperm and
offspring. We show that epigenetic inheritance of aberrant development
can be initiated by histone demethylase activity in developing sperm,
without changes to DNA methylation at CpG-rich regions.
|
| |
|
| Overall design |
Identification of the RNA population in sperm of wildtype mice, KDM1A transgenic mice and their non-transgenic littermates using gene expression array.
|
| |
|
| Contributor(s) |
Siklenka K, Erkek S, Peters AH, Kimmins S |
| Citation(s) |
26449473 |
| |
| Submission date |
Feb 18, 2015 |
| Last update date |
Feb 21, 2018 |
| Contact name |
Sarah Kimmins |
| E-mail(s) |
sarah.kimmins@mcgill.ca
|
| Organization name |
McGill University
|
| Street address |
21,111 Rue Lakesshore
|
| City |
Ste-anne-de-Bellevue |
| State/province |
Quebec |
| ZIP/Postal code |
H9X3V9 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
|
| Samples (7)
|
|
| This SubSeries is part of SuperSeries: |
| GSE66052 |
Disruption of histone methylation in developing sperm impairs offspring health transgenerationally |
|
| Relations |
| BioProject |
PRJNA275782 |
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