|
|
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Apr 25, 2016 |
Title |
Effect of mannose-binding lectin (MBL) on pulmonary gene expression in response to ozone |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
|
Summary |
Ozone is a common pollutant and a potent oxidant in industrialized nations. The mechanisms of ozone-induced lung injury and differential susceptibility are not fully understood. Ozone-induced lung inflammation is mediated, in part, by the innate immune system. We hypothesized that mannose binding lectin (MBL), which has a central role in the activation of the complement pathway of innate immunity, is a necessary component of the pro-inflammatory events caused by ozone-mediated activation of the innate immune system. Wild-type (Mbl+/+) and MBL deficient (Mbl-/-) mice were exposed to ozone (0.3 ppm) for 24, 48, and 72 hours, and bronchoalveolar lavage fluid (BALF) was examined for inflammatory markers. Compared to Mbl+/+ mice, significantly greater mean BALF eosinophils, neutrophils and neutrophil attractants CXCL2 (MIP-2) and CXCL5 (LIX) were found in Mbl-/- mice exposed to ozone. Using genome-wide mRNA microarray analyses, we identified significant differences in expression response profiles and networks at baseline (e.g. NRF2 mediated oxidative stress response) and after exposure (e.g. humoral immune response) between Mbl+/+ and Mbl-/- mice. The microarray data were further analyzed using a pattern recognition analysis for Extracting Patterns and Identifying co-expressed Genes (EPIG), and discovered several informative differential response patterns and subsequent gene sets, including antimicrobial response and inflammatory response. These novel findings demonstrate that targeted deletion of Mbl caused differential expression of inflammation-related gene sets basally and after exposure to ozone, and significantly reduced pulmonary inflammation thus indicating an important innate immunomodulatory role of the gene in this model.
|
|
|
Overall design |
Gene expression was measured in lungs of wild type or MBL knockout mice exposed to filtered air or ozone (0.3ppm) for 24, 48, or 72 hours.
|
|
|
Contributor(s) |
Kleeberger SR, Ciencewicki J, Verhein KC |
Citation(s) |
27106289 |
|
Submission date |
May 04, 2015 |
Last update date |
May 10, 2018 |
Contact name |
NIEHS Microarray Core |
E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
Organization name |
NIEHS
|
Department |
DIR
|
Lab |
Microarray Core
|
Street address |
111 T.W. Alexander Drive
|
City |
RTP |
State/province |
NC |
ZIP/Postal code |
27709 |
Country |
USA |
|
|
Platforms (1) |
GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
|
Samples (39)
|
|
Relations |
BioProject |
PRJNA282970 |
Supplementary file |
Size |
Download |
File type/resource |
GSE68522_RAW.tar |
359.0 Mb |
(http)(custom) |
TAR (of TXT) |
Processed data included within Sample table |
Processed data provided as supplementary file |
|
|
|
|
|