|
Status |
Public on May 15, 2015 |
Title |
Gene expression in IL-17RA-deficient and wild-type mice following Giardia muris infection |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
|
Summary |
To elucidate IL-17A-dependent immune mechanims involved in regulating host defense, we employed whole genome microarray expression profiling as a discovery platform to identify genes with the potential of regulating protective immunity that failed to be upregulated in the absence of IL-17RA signaling. Whole small intestine tissue collected from IL-17RA-deficient and C57BL/6 (wild-type) mice was collected 2 weeks after Giardia muris infection and from uninfected controls. Expression levels of several genes that may have anti-parasitic potential (Defb1, Retnlb, Saa1, Saa2) and may be involved in parasite clearance were, on average, lower or unchanged in IL-17RA deficient compared to wild-type mice after infection.
|
|
|
Overall design |
Two weeks after Giardia muris challenge, total RNA was extracted and analyzed from tissue of the small intestines of infected IL-17RA-deficient and wild-type mice, and compared to uninfected controls. Each sample contained equal amounts of total RNA from 6 female mice which were pooled and used in the experiment.
|
|
|
Contributor(s) |
Dann SM, Eckmann L |
Citation(s) |
26071205 |
|
Submission date |
May 14, 2015 |
Last update date |
May 10, 2018 |
Contact name |
Sara M Dann |
E-mail(s) |
smdann@utmb.edu
|
Organization name |
University of Texas Medical Branch
|
Department |
Internal Medicine
|
Street address |
301 University Blvd
|
City |
Galveston |
State/province |
TX |
ZIP/Postal code |
77555-0435 |
Country |
USA |
|
|
Platforms (1) |
GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
|
Samples (4)
|
|
Relations |
BioProject |
PRJNA283945 |