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| Status |
Public on Jan 11, 2016 |
| Title |
Pioneer factor FOXA1 defines GATA2- and AR-mediated transcriptional program in prostate cancer [ChIP-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
AR is tightly regulated by many transcriptional cofactors, including key pioneer factor such as FOXA1 and GATA2. While FOXA1 was recently shown to be able to redistribute AR across the genome, how GATA2 regulates AR cistrome has not been carefully investigated. Here, we report that, unlike FOXA1, GATA2 is unable to reprogram AR, but instead it enhances AR program by inducing AR expression and augmenting AR co-occupancy, thereby acting as a pure AR coactivator, rather than a pioneer factor. On the other hand, AR co-occupancy enhances both GATA2 and FOXA1 binding on the chromatin, forming a positive feedback loop. Importantly, we found that FOXA1 is also capable of reprogramming GATA2 by recruiting GATA2 from GATA motif to FKHD-containing regions, being analogous to its pioneering effect on AR signaling. By contrast, GATA2 simply acts as a co-activator of FOXA1 with a lack of pioneering ability.
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| Overall design |
ChIP_Seq examination of AR, FoxA1 and GATA2 binding sites in LNCaP and DU145 cells
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| Contributor(s) |
Zhao J, Yu J |
| Citation(s) |
26751772 |
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| Submission date |
May 19, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Jindan Yu |
| E-mail(s) |
jindan.yu@emory.edu
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| Organization name |
Emory University
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| Department |
Urology
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| Lab |
Jindan Yu's lab
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| Street address |
E330, 1760 Haygood Dr NE
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| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
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| Platforms (1) |
| GPL15456 |
Illumina HiScanSQ (Homo sapiens) |
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| Samples (28)
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| GSM1691138 |
LNCaP GIPZ RPMI+10%FBS AR ChIP m11 |
| GSM1691139 |
LNCaP shFOXA1 RPMI+10%FBS AR ChIP m12 |
| GSM1691140 |
LNCaP GIPZ phenolfree RPMI+5%FCS AR ChIP m13 |
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| This SubSeries is part of SuperSeries: |
| GSE69045 |
Pioneer factor FOXA1 defines GATA2- and AR-mediated transcriptional program in prostate cancer |
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| Relations |
| BioProject |
PRJNA284390 |
| SRA |
SRP058498 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE69043_RAW.tar |
1.4 Gb |
(http)(custom) |
TAR (of BEDGRAPH, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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