|
| Status |
Public on Jan 11, 2016 |
| Title |
DU145 CMV-AR Ad GATA2 AR ChIP m123 |
| Sample type |
SRA |
| |
|
| Source name |
DU145 CMV-AR Ad GATA2 AR ChIP m123
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: DU145
cell type: prostate tumor
treatment: Ad GATA2
antibody: AR (millipore, catalog# 06-680 lot# JBC1860890)
|
| Treatment protocol |
Chromatin was fixed in 1% formaldehyde, sheared to 200-700bp using an ultrasonic sonicator (Misonix), immunoprecipitated using the indicated antisera, purified and sequenced using Illumina Genome Analyzers followig protocols recommended by the manufacturer.
|
| Growth protocol |
Cell lines were maintained in RPMI supplemented with 10% FBS.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's instructions.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiScanSQ |
| |
|
| Description |
Chromatin IP against AR
|
| Data processing |
Reads were aligned to the hg19 reference human genome using bwa v0.6.1
Peak-calling by HOMER v4.1
Genome_build: hg19
Supplementary_files_format_and_content: bedGraph file and peak txt file
|
| |
|
| Submission date |
May 19, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Jindan Yu |
| E-mail(s) |
jindan.yu@emory.edu
|
| Organization name |
Emory University
|
| Department |
Urology
|
| Lab |
Jindan Yu's lab
|
| Street address |
E330, 1760 Haygood Dr NE
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platform ID |
GPL15456 |
| Series (2) |
| GSE69043 |
Pioneer factor FOXA1 defines GATA2- and AR-mediated transcriptional program in prostate cancer [ChIP-Seq] |
| GSE69045 |
Pioneer factor FOXA1 defines GATA2- and AR-mediated transcriptional program in prostate cancer |
|
| Relations |
| BioSample |
SAMN03701840 |
| SRA |
SRX1033289 |
