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Series GSE69080 Query DataSets for GSE69080
Status Public on Feb 26, 2016
Title Comprehensive Epigenomic Analysis Reveals Dynamic Regulatory Programs Of Blood Development (RNA-seq)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Embryonic hematopoiesis is regulated by the coordinated interaction between transcription factors and the epigenetic regulators driving developmental-stage specific gene expression but how this process drives hematopoietic specification and terminal differentiation is poorly understood. Here we generated RNA-Seq, DNase-Seq and ChIP-Seq data for histone marks and transcription factors from ES-cell derived purified cells representing six sequential stages of blood cell specification and differentiation. Our data reveal the binding patterns of specific transcription factors involved in the priming and maintenance of distal elements and inform how binding impacts on promoter activity. Functional studies based on these data uncovered a previously unrecognised role for Hippo signalling in mammalian hematopoietic specification. Finally, we present a dynamic core regulatory network model for hematopoiesis and demonstrate its utility for the design of reprogramming experiments. Our study represents a powerful resource for studying hematopoiesis and demonstrates how such data can advance our understanding of mammalian development.
Overall design ChIP-seq data of histone modifications and transcription factors, and RNA-seq data obtained from purified cells representing five sequential stages of murine blood cell specification and differentiation. In the expression cufflink file linked at the bottom of the Series record, there is processed data for a HE Sample, extensively used in this study, but has been uploaded previously in Series GSE55310.
Contributor(s) Goode DK, Obier N, Vijayabaskar M, Lichtinger M, Lie-A-Ling M, Hannah R, Lilly AJ, Florowska M, Batta K, Challinor M, Wallace K, Assi SA, Cauchy P, Gilmour J, Hoogenkamp M, Westhead DR, Lacaud G, Kouskoff V, Göttgens B, Bonifer C
Citation(s) 26923725, 29530939
Submission date May 20, 2015
Last update date Jul 29, 2019
Contact name MS Vijayabaskar
Organization name University of Leeds
Department School of Molecular and Cellular Biology
Street address Woodhouse Lane
City Leeds
ZIP/Postal code LS2 9JT
Country United Kingdom
Platforms (2)
GPL15907 AB 5500xl Genetic Analyzer (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (18)
GSM1692623 MES_RNASeq_Rep1
GSM1692624 MES_RNASeq_Rep2
GSM1692625 HB_RNASeq_Rep1
This SubSeries is part of SuperSeries:
GSE69101 Comprehensive Epigenomic Analysis Reveals Dynamic Regulatory Programs Of Blood Development
Reanalysis of GSM1333919
Reanalysis of GSM1333920
BioProject PRJNA284510
SRA SRP058533

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE69080_expression_cufflinks.txt.gz 838.0 Kb (ftp)(http) TXT
GSE69080_reprogrna_expression_cufflinks.txt.gz 565.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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