|
| Status |
Public on Feb 25, 2016 |
| Title |
21_SL |
| Sample type |
SRA |
| |
|
| Source name |
Mouse Embryonic Fibroblasts
|
| Organism |
Mus musculus |
| Characteristics |
strain: 129
cell type: MEF
genotype/variation: MEFs (Scl/Lmo2) (21 days)
|
| Growth protocol |
Embryonic day 14.5 murine wild type fibroblasts (MEFs) or from iTal1-2A-GFP transgenic mouse carrying rtTA and TRE-TAL1 cassettes allowing inducible transgenic mouse carrying rtTA and TRE-TAL1 cassettes allowing inducible expression of TAL1 upon addition of doxycyclin were prepared as described before (Sroczynska et al., 2009). Reprogramming experiments were carried out with either wild type (n=5) or iTal1-2A-GFP (n=2) MEFs as described in (Batta et al., 2014)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA preparation and sequencing were performed as described previously (Lie-A-Ling et al 2014).
RNA-seq libraries were generated with the Total RNA-Seq Kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Aligned using TopHat
Expression analysis using Cufflinks
Genome_build: mm10
Supplementary_files_format_and_content: fastq (unmapped reads), txt (gene expression)
|
| |
|
| Submission date |
Dec 09, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
MS Vijayabaskar |
| E-mail(s) |
fbsvbm@leeds.ac.uk
|
| Organization name |
University of Leeds
|
| Department |
School of Molecular and Cellular Biology
|
| Street address |
Woodhouse Lane
|
| City |
Leeds |
| ZIP/Postal code |
LS2 9JT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE69080 |
Comprehensive Epigenomic Analysis Reveals Dynamic Regulatory Programs Of Blood Development (RNA-seq) |
| GSE69101 |
Comprehensive Epigenomic Analysis Reveals Dynamic Regulatory Programs Of Blood Development |
|
| Relations |
| BioSample |
SAMN04328096 |
| SRA |
SRX1472997 |
