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| Status |
Public on May 03, 2016 |
| Title |
Gene expression profile of F4/80+ cells isolated from tumors implanted in wild type and St2-/- mouse |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We report a novel mechanism of the interaction between perivascular cell and TAMs in promoting metastasis through the IL-33-ST2-dependent pathway. IL-33 was the highest up-regulated gene through activation of SOX7 transcription factor in PDGF-BB-stimulated pericytes. Gain- and loss-of-function experiments validate that IL-33 promotes metastasis through recruitment of TAMs. Il33-/- deficient mice showed impaired TAM recruitment and metastasis. Pharmacological inhibition of the IL-33-ST2 signalling by a soluble ST2 significantly inhibited TAMs and metastasis. Genetic deletion of host IL-33 in mice also blocked PDGF-BB-induced TAM recruitment and metastasis. High IL-33 in human cancers correlated with poor survival prognosis. These findings shed novel mechanisms of tumour stroma in promoting metastasis and have therapeutic implications for cancer therapy.
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| |
|
| Overall design |
F4/80+ cells are isolated from tumors implanted in wild type and St2-/- mouse (n=3 samples/group)
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| |
|
| Contributor(s) |
Yang Y, Cao Y |
| Citation(s) |
30333314, 27150562 |
| |
| Submission date |
May 31, 2015 |
| Last update date |
Jul 18, 2019 |
| Contact name |
Hiromasa Morikawa |
| E-mail(s) |
hiromasa.morikawa@ki.se
|
| Phone |
+46762923821
|
| Organization name |
Karolinska Institutet
|
| Department |
Medicine
|
| Lab |
Unit of Computational Medicine
|
| Street address |
Karolinska University Hospital, L8:05
|
| City |
Stockholm |
| State/province |
Stockholms |
| ZIP/Postal code |
171 76 |
| Country |
Sweden |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (6)
|
| GSM1700738 |
F4/80+ cells from tumors implanted in wild, rep1 |
| GSM1700739 |
F4/80+ cells from tumors implanted in wild, rep2 |
| GSM1700740 |
F4/80+ cells from tumors implanted in wild, rep3 |
| GSM1700741 |
F4/80+ cells from tumors implanted in St2-/-, rep1 |
| GSM1700742 |
F4/80+ cells from tumors implanted in St2-/-, rep2 |
| GSM1700743 |
F4/80+ cells from tumors implanted in St2-/-, rep3 |
|
| Relations |
| BioProject |
PRJNA285414 |
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