|
Status |
Public on May 03, 2016 |
Title |
Gene expression profile of F4/80+ cells isolated from tumors implanted in wild type and St2-/- mouse |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
|
Summary |
We report a novel mechanism of the interaction between perivascular cell and TAMs in promoting metastasis through the IL-33-ST2-dependent pathway. IL-33 was the highest up-regulated gene through activation of SOX7 transcription factor in PDGF-BB-stimulated pericytes. Gain- and loss-of-function experiments validate that IL-33 promotes metastasis through recruitment of TAMs. Il33-/- deficient mice showed impaired TAM recruitment and metastasis. Pharmacological inhibition of the IL-33-ST2 signalling by a soluble ST2 significantly inhibited TAMs and metastasis. Genetic deletion of host IL-33 in mice also blocked PDGF-BB-induced TAM recruitment and metastasis. High IL-33 in human cancers correlated with poor survival prognosis. These findings shed novel mechanisms of tumour stroma in promoting metastasis and have therapeutic implications for cancer therapy.
|
|
|
Overall design |
F4/80+ cells are isolated from tumors implanted in wild type and St2-/- mouse (n=3 samples/group)
|
|
|
Contributor(s) |
Yang Y, Cao Y |
Citation(s) |
30333314, 27150562 |
|
Submission date |
May 31, 2015 |
Last update date |
Jul 18, 2019 |
Contact name |
Hiromasa Morikawa |
E-mail(s) |
hiromasa.morikawa@ki.se
|
Phone |
+46762923821
|
Organization name |
Karolinska Institutet
|
Department |
Medicine
|
Lab |
Unit of Computational Medicine
|
Street address |
Karolinska University Hospital, L8:05
|
City |
Stockholm |
State/province |
Stockholms |
ZIP/Postal code |
171 76 |
Country |
Sweden |
|
|
Platforms (1) |
GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
Samples (6)
|
GSM1700738 |
F4/80+ cells from tumors implanted in wild, rep1 |
GSM1700739 |
F4/80+ cells from tumors implanted in wild, rep2 |
GSM1700740 |
F4/80+ cells from tumors implanted in wild, rep3 |
GSM1700741 |
F4/80+ cells from tumors implanted in St2-/-, rep1 |
GSM1700742 |
F4/80+ cells from tumors implanted in St2-/-, rep2 |
GSM1700743 |
F4/80+ cells from tumors implanted in St2-/-, rep3 |
|
Relations |
BioProject |
PRJNA285414 |