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| Status |
Public on Jun 10, 2015 |
| Title |
Gene expression profiles of induced pluripotent stem cells (iPSCs) and skin fibroblasts from orangutans |
| Platform organism |
Homo sapiens |
| Sample organism |
Pongo abelii |
| Experiment type |
Expression profiling by array
|
| Summary |
Orangutans are an endangered species whose natural habitats are restricted to the Southeast Asian islands of Borneo and Sumatra. For potential species conservation and functional genomics studies, we derived induced pluripotent stem cells (iPSCs) from cryopreserved skin fibroblasts obtained from captive orangutans.
We report the gene expression profiles of iPSCs and skin fibroblasts derived from orangtuans.
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| Overall design |
The overall goal was to evaluate gene expression biomarkers of pluripotency in iPSCs and skin fibroblasts derived from PBD-ZSD patients and healthy controls. Dermal fibroblast cultures from 2 orangutans were reprogrammed into iPSCs by transfection with retroviruses designed to express the human OCT4, SOX2, KLF4 and c-MYC cDNA. Fibroblasts and iPSCs were cultured in 1:1 ratio of DMEM:F12 medium supplemented with 20% KOSR (knock-out serum replacement) at 37°C with 5% CO2 until confluence for RNA extraction. The overall goal was to evaluate gene expression biomarkers of pluripotency in iPSCs and original fibroblast cultures.
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| Contributor(s) |
Wang X, Hacia JG |
| Citation(s) |
26475477 |
| |
| Submission date |
Jun 05, 2015 |
| Last update date |
Dec 06, 2018 |
| Contact name |
Joseph Gerard Hacia |
| E-mail(s) |
hacia@hsc.usc.edu
|
| Phone |
323-442-3030
|
| Organization name |
University of Southern California
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Hacia Lab
|
| Street address |
2250 Alcazar Street, IGM 261
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90089 |
| Country |
USA |
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| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (8)
|
| GSM1704619 |
Cultured fibroblasts from orangutan KB10460 |
| GSM1704620 |
Cultured fibroblasts from orangutan KB10973 |
| GSM1704621 |
Cultured iPSC colony 1 from orangutan KB10460 |
| GSM1704622 |
Cultured iPSC colony 2 from orangutan KB10460 |
| GSM1704623 |
Cultured iPSC colony 3 from orangutan KB10460 |
| GSM1704624 |
Cultured iPSC colony 1 from orangutan KB10973 |
| GSM1704625 |
Cultured iPSC colony 2 from orangutan KB10973 |
| GSM1704626 |
Cultured iPSC colony 3 from orangutan KB10973 |
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| Relations |
| BioProject |
PRJNA285960 |
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