|
| Status |
Public on Jun 10, 2015 |
| Title |
Cultured iPSC colony 2 from orangutan KB10460 |
| Sample type |
RNA |
| |
|
| Source name |
iPSC from Sumatran orangutan KB10460
|
| Organism |
Pongo abelii |
| Characteristics |
tissue: induced pluripotent stem cells
genotype: healthy control
age: 43 year old
Sex: female
|
| Treatment protocol |
Fibroblasts were dissociated with 0.05% trypsin at 37°C, 5% CO2 for 5minutes and collected by centrifugation; iPSCs were dissociated with 1mg/ml collagenase IV at 37°C, 5% CO2 for 5 minutes, manually detached and collected by centrifugation
|
| Growth protocol |
Fibroblasts were maintained in Dulbecco’s modified eagle medium (DMEM) containing 10% fetal bovine serum (FBS), 2 mM L-glutamine, 100 µM nonessential amino acids and 0.5% penicillin and streptomycin. iPSCs were cultured in 1:1 ratio of DMEM:F12 medium supplemented with 20% KSR at 37°C, 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated using the RNA Stat-60 reagent according to the manusfacturer's suggested protocol (Tel-Test, Inc.).
|
| Label |
biotin
|
| Label protocol |
followed affymetrix protocol
|
| |
|
| Hybridization protocol |
followed affymetrix protocol
|
| Scan protocol |
followed affymetrix protocol
|
| Description |
Gene expression data of orangutan KB10460 iPSC colony 2
|
| Data processing |
WebArray software was used to generate log2-transformed gene expression values using the RMA algorithm
|
| |
|
| Submission date |
Jun 05, 2015 |
| Last update date |
Jun 10, 2015 |
| Contact name |
Joseph Gerard Hacia |
| E-mail(s) |
hacia@hsc.usc.edu
|
| Phone |
323-442-3030
|
| Organization name |
University of Southern California
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Hacia Lab
|
| Street address |
2250 Alcazar Street, IGM 261
|
| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90089 |
| Country |
USA |
| |
|
| Platform ID |
GPL571 |
| Series (1) |
| GSE69603 |
Gene expression profiles of induced pluripotent stem cells (iPSCs) and skin fibroblasts from orangutans |
|
