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Status |
Public on Oct 29, 2015 |
Title |
Gene expression profiles of normal human tonsillar cell subpopulations FACS sorted based on expression of cell surface markers |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
To characterize the progenitor-enriched CD44+NGFR+ cells present in the crypt and surface regions of the tonsil, we used an Illumina array system to analyze the transcriptome of these cells isolated from the tonsillar crypt and surface regions of three different individuals as well as the total epithelial (CD45-CD31-) populations obtained from two of these.
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Overall design |
To enrich for epithelial progeniors in human palatine tonsil tissue, hematopoietic and endothelial cells were depleted from freshly isolated cell suspensions derived from palatine tonsils using fluorescence-activated cell sorting (FACS). The resultant CD45-CD31- population was further fractionated into four subpopulations using antibodies against CD44 and NGFR. Based on the immunohistochemical phenotype and in vitro functional assays, CD44+NGFR+ subpopulations were identified as epithelial progenitor-enriched subsets. Microarray profiling was used to derive gene expression signatures of CD45-CD31- subsets (total epithelial-enriched) and CD45-CD31-CD44+NGFR+ subpopulations (progenitor-enriched).
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Contributor(s) |
Kang SC |
Citation(s) |
26527383 |
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Submission date |
Jun 11, 2015 |
Last update date |
Aug 13, 2018 |
Contact name |
Catherine Kang |
E-mail(s) |
ckang@bccrc.ca
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Organization name |
British Columbia Cancer Research Centre
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Street address |
675 West 10th Avenue
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City |
Vancouver |
State/province |
British Columbia |
ZIP/Postal code |
V5Z 1L3 |
Country |
Canada |
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Platforms (1) |
GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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Samples (10)
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Relations |
BioProject |
PRJNA286736 |