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Series GSE69767 Query DataSets for GSE69767
Status Public on Oct 29, 2015
Title Gene expression profiles of normal human tonsillar cell subpopulations FACS sorted based on expression of cell surface markers
Organism Homo sapiens
Experiment type Expression profiling by array
Summary To characterize the progenitor-enriched CD44+NGFR+ cells present in the crypt and surface regions of the tonsil, we used an Illumina array system to analyze the transcriptome of these cells isolated from the tonsillar crypt and surface regions of three different individuals as well as the total epithelial (CD45-CD31-) populations obtained from two of these.
 
Overall design To enrich for epithelial progeniors in human palatine tonsil tissue, hematopoietic and endothelial cells were depleted from freshly isolated cell suspensions derived from palatine tonsils using fluorescence-activated cell sorting (FACS). The resultant CD45-CD31- population was further fractionated into four subpopulations using antibodies against CD44 and NGFR. Based on the immunohistochemical phenotype and in vitro functional assays, CD44+NGFR+ subpopulations were identified as epithelial progenitor-enriched subsets. Microarray profiling was used to derive gene expression signatures of CD45-CD31- subsets (total epithelial-enriched) and CD45-CD31-CD44+NGFR+ subpopulations (progenitor-enriched).
 
Contributor(s) Kang SC
Citation(s) 26527383
Submission date Jun 11, 2015
Last update date Aug 13, 2018
Contact name Catherine Kang
E-mail(s) ckang@bccrc.ca
Organization name British Columbia Cancer Research Centre
Street address 675 West 10th Avenue
City Vancouver
State/province British Columbia
ZIP/Postal code V5Z 1L3
Country Canada
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (10)
GSM1708560 tonsil_prog_1_surface
GSM1708561 tonsil_prog_1_crypt
GSM1708562 tonsil_prog_2_surface
Relations
BioProject PRJNA286736

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE69767_RAW.tar 26.2 Mb (http)(custom) TAR
GSE69767_non-normalized.txt.gz 3.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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