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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 31, 2018 |
| Title |
Bone marrow neutrophils regulate multiple myeloma by releasing DNA |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Development of Multiple Myeloma is associated with an accumulation of myeloid derived suppressor cells (MDSCs ) - cells that are morphologically and phenotypically similar to neutrophils (PMN-MDSC) and monocytes (M-MDSC), but are distinct in their functional and biochemical characteristics as well as in their ability to suppress immune responses.
In our study, we found that both neutrophils and PMN-MDSCs equally promoted MM survival and decreased MM cell sensitivity to chemotherapy. Therefore, we used a microarray to elucidate the mechanism of the protective effect of neutrophils on myeloma cells.
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| Overall design |
DP42 murine myeloma cells were cultured alone or together with mouse B-cells (control) or neutrophils for 24 hours. The cells were subsequently separated using FACs and RNA was extracted using Trizol
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| Contributor(s) |
Ramachandran I, Lin C, Nefedova Y |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
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| Submission date |
Jun 12, 2015 |
| Last update date |
Sep 01, 2018 |
| Contact name |
Cindy Lin |
| E-mail(s) |
clin@wistar.org
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| Organization name |
Wistar Institute
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| Lab |
Nefedova Lab
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| Street address |
3601 Spruce Street
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA286889 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE69834_RAW.tar |
60.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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