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Series GSE70455 Query DataSets for GSE70455
Status Public on Aug 01, 2017
Title Dynamic of DNA methylation during Arabidopsis reproduction
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary Cytosine methylation is a key epigenetic mark in many organisms, important for both transcriptional control and genome integrity. While relatively stable during somatic growth, DNA methylation is reprogrammed genome-wide during mammalian reproduction. Reprogramming is essential for zygotic totipotency, and to prevent transgenerational inheritance of epimutations. The extent of DNA methylation reprogramming in plants however remains unclear. Here, we developed sensors reporting with single-cell resolution CG and non-CG methylation in Arabidopsis. Live imaging during reproduction revealed distinct and sex-specific dynamics for both contexts. We found that CHH methylation in the egg cell depends on DRM2 and Pol V, two main actors of RNA-directed DNA methylation, but does not depend on Pol IV. Our sensors provide insight into global DNA methylation dynamics at the single cell level with high temporal resolution, and offer a powerful tool to track CG and non-CG methylation both during development and in response to environmental cues in all organisms with methylated DNA.

Overall design ChIP-seq experiments conducted using transgenic lines expressing either CG or non-CG binding proteins. mCG in WT (Col(O)), mCHH and drm1-drm2-cmt2-cmt3, plus inputs.
Contributor(s) Grimanelli D, Ingouff M
Citation(s) 28115468
Submission date Jul 01, 2015
Last update date May 15, 2019
Contact name Daniel Grimanelli
Phone +33 4 67 41 63 76
Organization name IRD
Lab Epigenetics and Seed Development Lab
Street address 911 Av Agropolis
City Montpellier
ZIP/Postal code 34394
Country France
Platforms (2)
GPL13222 Illumina HiSeq 2000 (Arabidopsis thaliana)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
Samples (19)
GSM1755129 mCG_protocol_validation_chip
GSM1755130 mCG rep2
GSM1755131 mCHH rep1
BioProject PRJNA288783
SRA SRP060240

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Supplementary file Size Download File type/resource
GSE70455_RAW.tar 2.0 Gb (http)(custom) TAR (of BED)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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