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| Status |
Public on Jan 31, 2016 |
| Title |
Adaptive Evolution and Regulation of a Stress-Activated Transposon in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
|
| Summary |
To understand an effect of an ONSEN insertion for a gene network, a genome wide gene expression on the ONSEN inserted lines was analyzed by a microarray. The microarray analysis identified 6,520 and 7,262 significant different expressed genes (3/2 fold or 2/3 fold, FDR < 0.05) in 13-7 and 19-4 respectively under ABA stress.
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| Overall design |
Two of ONSEN inserted lines (13-7, 19-4), parent line (nrpd1), and wild-type plants (Arabidopsis thaliana ecotype Columbia) were grown on MS medium with and without 2μM ABA for 7 days. Total RNA was extructed from the plants, using TRI Reagent (Sigma T9424). 100 ng of the isolated tatal RNAs were used for the expresion profiling using the custom array.
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| Web link |
http://www.nature.com/articles/srep23181
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| |
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| Contributor(s) |
Ito H, Matsui A, Tanaka M, Iida K, Seki M |
| Citation(s) |
26976262 |
| |
| Submission date |
Aug 11, 2015 |
| Last update date |
May 05, 2016 |
| Contact name |
Akihiro Matsui |
| E-mail(s) |
akihiro.matsui@riken.jp
|
| Phone |
+81-45-503-9587
|
| Organization name |
RIKEN
|
| Department |
Center for Sustainable Resource Science
|
| Lab |
Plant Genomic Network Research Team
|
| Street address |
1-7-22 Suehiro-cho, Tsurumi-ku
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
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| Platforms (1) |
| GPL20796 |
Agilent-034592 Arabidopsis thaliana PSC_Custom |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA292558 |
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