|
| Status |
Public on Jan 31, 2016 |
| Title |
13-7_con_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
13-7 without ABA
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Columbia
line: 13-7
|
| Treatment protocol |
MS medium plates containg 2μM abscisic acid (ABA)
|
| Growth protocol |
Two of ONSEN inserted lines (13-7, 19-4), parent line (nrpd1), and wild-type plants (Arabidopsis thaliana ecotype Columbia) were grown on MS medium with and without 2μM ABA for 7 days
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from 7 day-old seedlings using TRI Reagent (Sigma T9424), according to the supplier’s recommendations.
|
| Label |
Cy3
|
| Label protocol |
100 ng of total RNA samples were used to prepare for Cy3-labeled cRNA, using Low Input Quick Amp Labeling Kit (Agilent).
|
| |
|
| Hybridization protocol |
600 ng of cRNAs were hybridized to the microarray (GPL19830) at 65 degree for 17 hours, using Gene Expression Hybridization kit. After hybridization, the microarray was washed with Gene Expression Wash Buffer 1 (Agilent) and Gene Expression Wash Buffer (Agilent), and then dried immediately by brief centrifugation.
|
| Scan protocol |
The microarray was scanned by Agilent DNA Microarray Scanner G2539A ver. C and raw data was extracted using Feature Extraction program ver 9.1.
|
| Description |
ONSEN inserted line
|
| Data processing |
The signals of microarray probes were applied to 75 percentile normalization
|
| |
|
| Submission date |
Aug 11, 2015 |
| Last update date |
Jan 31, 2016 |
| Contact name |
Akihiro Matsui |
| E-mail(s) |
akihiro.matsui@riken.jp
|
| Phone |
+81-45-503-9587
|
| Organization name |
RIKEN
|
| Department |
Center for Sustainable Resource Science
|
| Lab |
Plant Genomic Network Research Team
|
| Street address |
1-7-22 Suehiro-cho, Tsurumi-ku
|
| City |
Yokohama |
| State/province |
Kanagawa |
| ZIP/Postal code |
230-0045 |
| Country |
Japan |
| |
|
| Platform ID |
GPL20796 |
| Series (1) |
| GSE71951 |
Adaptive Evolution and Regulation of a Stress-Activated Transposon in Arabidopsis |
|
