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GEO help: Mouse over screen elements for information. |
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Status |
Public on Oct 12, 2007 |
Title |
Identification of cancer modifiers using parental strain expression mapping |
Organism |
Mus musculus |
Experiment type |
Expression profiling by array
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Summary |
Inherited genetic risk factors play an important role in cancer. However, other than cancer susceptibility genes found in familial cancer syndromes and inherited in a Mendelian fashion, little is known about modifier genes (germline variants that interact with each other and with environmental factors) that contribute to individual susceptibility. Here we develop a strategy “parental strain expression mapping” (PSEM), which utilizes the homogeneity of inbred mice and genome-wide mRNA expression analyses, to directly identify candidate germline modifier genes and pathways underlying phenotypic differences among murine strains exposed to transgenic activation of AKT1. We identified multiple candidate modifier pathways and specifically, the glycolysis pathway as a candidate negative modulator of AKT1-induced proliferation. In keeping with findings in murine models, the expression of the glycolysis pathway was strongly enriched in the non-cancer prostate tissue from patients with prostate cancer who did not recur after surgical resection. Together these data suggest that PSEM can directly identify germline modifier pathways of relevance to human disease. Keywords: genetic modifier
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Overall design |
mRNA expression profiling was carried out with 24 samples of VP from 6 inbred strains.
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Contributor(s) |
Xu Q, Ross K, Sellers W |
Citation(s) |
17978178 |
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Submission date |
Mar 14, 2007 |
Last update date |
Jan 08, 2019 |
Contact name |
qing xu |
E-mail(s) |
qing_xu@dfci.harvard.edu
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Phone |
6176322930
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Organization name |
dana farber cancer institute
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Department |
Medical Oncology
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Lab |
sellers
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Street address |
44 binney st
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City |
boston |
State/province |
MA |
ZIP/Postal code |
02115 |
Country |
USA |
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Platforms (1) |
GPL339 |
[MOE430A] Affymetrix Mouse Expression 430A Array |
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Samples (24)
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GSM175345 |
AKR VP, biological rep4 |
GSM175346 |
SWR VP, biological rep1 |
GSM175347 |
SWR VP, biological rep2 |
GSM175348 |
SWR VP, biological rep3 |
GSM175349 |
SWR VP, biological rep4 |
GSM175350 |
129X1 VP, biological rep1 |
GSM175351 |
129X1 VP, biological rep2 |
GSM175352 |
129X1 VP, biological rep3 |
GSM175353 |
129X1 VP, biological rep4 |
GSM175354 |
FVB VP, biological rep1 |
GSM175355 |
FVB VP, biological rep2 |
GSM175356 |
FVB VP, biological rep3 |
GSM175357 |
FVB VP, biological rep4 |
GSM175358 |
BALB/c VP, biological rep1 |
GSM175359 |
BALB/c VP, biological rep2 |
GSM175360 |
BALB/c VP, biological rep3 |
GSM175361 |
BALB/c VP, biological rep4 |
GSM175362 |
C57BL/6 VP, biological rep1 |
GSM175363 |
C57BL/6 VP, biological rep2 |
GSM175364 |
C57BL/6 VP, biological rep3 |
GSM175365 |
C57BL/6 VP, biological rep4 |
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Relations |
BioProject |
PRJNA97987 |
Supplementary file |
Size |
Download |
File type/resource |
GSE7270_RAW.tar |
80.9 Mb |
(http)(custom) |
TAR (of CEL) |
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