|
| Status |
Public on Jan 09, 2016 |
| Title |
Gene expression analysis of untreated and NT-shRNA transduced kidney proximal tubular epithelial cells (PTECs) at day 6 and day 16 after transduction, as well as SETD2-shRNA transduced PTECs at day 25 after transduction |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Primary kidney PTECs gradually became senescent after a few passages, but SETD2 depletion prevented PTECs from senescence and maintained their proliferation beyond their limited dividing capacity. Transcriptional profiling of human PTECs, with comparing of SETD2 wild-type non-senescent PTECs at day 6 (WT-PTECs-day 6), SETD2 wild-type senescent PTECs at day 16 (WT-PTECs-day16), and SETD2 knockdown PTECs at day 25 (KD-PTECs-day 25).
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| Overall design |
Three PTECs of different origins were transduced with shRNA constructs against SETD2 (sh1 or sh2), or with a non-targeting sequence. Untreated and NT-shRNA transduced samples were harvest at day 6 and day 16 respectively, SETD2-KD shRNA transduced PTECs were harvest at day 25.
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| Contributor(s) |
Li J, Kok K, van den Berg A, Sijmons RH, Westers H, Kluiver J, Osinga J, van Werkhoven MB, Seelen MA, de Jong D, Terpstra M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 08, 2015 |
| Last update date |
Oct 27, 2016 |
| Contact name |
Jun Li |
| E-mail(s) |
nexusoooooo@gmail.com
|
| Phone |
+31641112670
|
| Organization name |
University Medical Center Groningen
|
| Department |
Genetics
|
| Lab |
Klaas Kok
|
| Street address |
Esdoornlaan
|
| City |
GRONINGEN |
| State/province |
Netherlands |
| ZIP/Postal code |
9741MG |
| Country |
Netherlands |
| |
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| Platforms (1) |
| GPL18641 |
Agilent-050524 CMGG Human V2.0 60k |
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| Samples (18)
|
| GSM2121041 |
non-treated primary kidney proximal tubular epithelial cells at day 6 replicate1 |
| GSM2121042 |
non-treated primary kidney proximal tubular epithelial cells at day 6 replicate2 |
| GSM2121043 |
non-treated primary kidney proximal tubular epithelial cells at day 6 replicate3 |
| GSM2121044 |
NT-shRNA treated primary kidney proximal tubular epithelial cells at day 6 replicate1 |
| GSM2121045 |
NT-shRNA treated primary kidney proximal tubular epithelial cells at day 6 replicate2 |
| GSM2121046 |
NT-shRNA treated primary kidney proximal tubular epithelial cells at day 6 replicate3 |
| GSM2121047 |
non-treated primary kidney proximal tubular epithelial cells at day 16 replicate1 |
| GSM2121048 |
non-treated primary kidney proximal tubular epithelial cells at day 16 replicate2 |
| GSM2121049 |
non-treated primary kidney proximal tubular epithelial cells at day 16 replicate3 |
| GSM2121050 |
NT-shRNA treated primary kidney proximal tubular epithelial cells at day 16 replicate1 |
| GSM2121051 |
NT-shRNA treated primary kidney proximal tubular epithelial cells at day 16 replicate2 |
| GSM2121052 |
NT-shRNA treated primary kidney proximal tubular epithelial cells at day 16 replicate3 |
| GSM2121053 |
sh1-shRNA treated primary kidney proximal tubular epithelial cells at day 25 replicate1 |
| GSM2121054 |
sh1-shRNA treated primary kidney proximal tubular epithelial cells at day 25 replicate2 |
| GSM2121055 |
sh1-shRNA treated primary kidney proximal tubular epithelial cells at day 25 replicate3 |
| GSM2121056 |
sh2-shRNA treated primary kidney proximal tubular epithelial cells at day 25 replicate1 |
| GSM2121057 |
sh2-shRNA treated primary kidney proximal tubular epithelial cells at day 25 replicate2 |
| GSM2121058 |
sh2-shRNA treated primary kidney proximal tubular epithelial cells at day 25 replicate3 |
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| Relations |
| BioProject |
PRJNA295076 |
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