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Series GSE73868 Query DataSets for GSE73868
Status Public on Feb 09, 2017
Title High throughput bisulfite sequencing of the MLH1 promoter in human hematiopoietic progenitor cell clones
Organism Homo sapiens
Experiment type Methylation profiling by high throughput sequencing
Summary Using single molecule ultra-deep bisulfite sequencing we characterized the CpG methylation landscape from –938 to –337 bp upstream of the MLH1 transcriptional start site position 0, from 30 hematopoietic colony forming cell clones (CFC) either expressing or not expressing MLH1. We identify a correlation between MLH1 promoter methylation and loss of MLH1 expression. Additionally, using the CpG site methylation frequencies obtained in this study we are able to generate a classification algorithm capable of sorting the expressing and non-expressing CFC. Thus, as previously described for many tumor cell types, MLH1 promoter methylation in hematopoietic stem cell clones correlates with the loss of MLH1 expression, a critically important gene in the mismatch repair pathway.
Overall design Examination of CpG methylation landscape upstream of the MLH1 transcriptional start site in human hematiopoietic progenitor cell clones
Contributor(s) Kenyon JD, Nickel G, Qing Y, Santos-Guasch G, Drake E, Fu P, Sun S, Bai X, Wald D, Arts E, Gerson SL
Citation(s) 27570841
Submission date Oct 08, 2015
Last update date May 15, 2019
Contact name Jonathan D Kenyon
Organization name Case Western Reserve University
Department Pathology
Lab 2-104 WRB
Street address 2103 Cornell
City Cleveland
State/province OH
ZIP/Postal code 44106
Country USA
Platforms (1)
GPL9186 454 GS FLX (Homo sapiens)
Samples (39)
GSM1904620 BMA03-T1C5
GSM1904621 BMA03-T2C5
GSM1904622 Methylated
BioProject PRJNA298249
SRA SRP064627

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE73868_RAW.tar 12.5 Mb (http)(custom) TAR (of CSV, TAB)
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Raw data are available in SRA
Processed data provided as supplementary file

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