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| Status |
Public on Jan 01, 2016 |
| Title |
Translational control of autophagy by Orb in the Drosophila germline [mature] |
| Organism |
Drosophila melanogaster |
| Experiment type |
Other
|
| Summary |
Drosophila Orb, the homologue of vertebrate CPEB is a key translational regulator involved in oocyte polarity and maturation through poly(A) tail elongation of specific mRNAs. orb has also an essential function during early oogenesis which has not been addressed at the molecular level. Here, we show that orb prevents cell death during early oogenesis, thus allowing oogenesis to progress. It does so through the repression of autophagy, by directly repressing, together with the CCR4 deadenylase, the translation of Autophagy-specific gene 12 (Atg12) mRNA. Autophagy and cell death observed in orb mutant ovaries are reduced by decreasing Atg12 or other Atg mRNA levels. These results reveal a role of Orb in translational repression and identify autophagy as an essential pathway regulated by Orb during early oogenesis. Importantly, they also establish translational regulation as a major mode of control of autophagy, a key process in cell homeostasis in response to environmental cues.
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| |
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| Overall design |
Orb RIP-chip vs mock IP on ovaries from mature 3-5 day old females.
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| Contributor(s) |
Busseau I, Séverac D, Dantec C, Pierson S, Rojas-Rios P, Chartier A, Simonelig M |
| Citation(s) |
26625957 |
| |
| Submission date |
Oct 29, 2015 |
| Last update date |
Apr 06, 2016 |
| Contact name |
Simonelig Martine |
| E-mail(s) |
martine.simonelig@igh.cnrs.fr
|
| Organization name |
Institut de Genetique Humaine - CNRS
|
| Department |
Genetics and Development
|
| Lab |
mRNA Regulation and Development
|
| Street address |
141, rue de la Cardonille
|
| City |
MONTPELLIER |
| ZIP/Postal code |
34396 |
| Country |
France |
| |
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| Platforms (1) |
| GPL5112 |
MLRG/DrosoIndac Drosophila melanogaster 15K |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE74476 |
Translational control of autophagy by Orb in the Drosophila germline |
|
| Relations |
| BioProject |
PRJNA300531 |
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