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| Status |
Public on Nov 06, 2015 |
| Title |
Gene transcription, metabolite and lipid profiling in eco-indicator daphnia magna indicate diverse mechanisms of toxicity by legacy and emerging flame-retardants. |
| Organism |
Daphnia magna |
| Experiment type |
Expression profiling by array
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| Summary |
The use of chemical flame-retardants (FR) in consumer products has steadily increased over the last 30 years. Toxicity data exist for legacy FRs such as pentabromodiphenyl ether (pentaBDE), but less is known about effects of new formulations. To address this issue, the toxicity of seven FR chemicals and formulations was assessed on the freshwater crustacean Daphnia magna. Acute 48-h nominal LC50 values for penta- and octabromodiphenyl ether (pentaBDE, octaBDE), Firemaster 550 (FM550), Firemaster BZ-54 (BZ54), bis(2-ethylhexyl) tetrabromophthalate (BEH-TEBP), triphenyl phosphate (TPhP), and nonbrominated BEH-TEBP analog bis(2-ethylhexyl) phthalate (BEHP) ranged from 0.058 mg/L (pentaBDE) to 3.96 mg/L (octaBDE). mRNA expression, (1)H NMR-based metabolomic and lipidomic profiling at 1/10 LC50 revealed distinct patterns of molecular response for each exposure, suggesting pentaPBDE affects transcription and translation, octaBDE and BEH-TEBP affect glycosphingolipid biosynthesis and BZ54 affects Wnt and Hedgehog signal pathways as well as glycosaminoglycan degradation. Brominated components of FM550 (i.e., BZ54) were significantly higher in Daphnia after 48 h following 1/10 LC50 exposure. FM550 elicited significant mRNA changes at five concentrations across a range from 1/10^6 LC50 to 1/2 LC50. Analyses suggest FM550 impairs nutrient utilization or uptake in Daphnia.
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| Overall design |
Seven flame retardant formulations and equal volume solvent (DMSO) controls for each; four exposed and three or four biological replicates per condition.
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| Contributor(s) |
Scanlan LD, Loguinov AV, Teng Q, Antczak P, Dailey KP, Nowinski DT, Kornbluh J, Lin XX, Lachenauer E, Arai A, Douglas NK, Falciani F, Stapleton HM, Vulpe CD |
| Citation(s) |
25985095 |
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| Submission date |
Nov 05, 2015 |
| Last update date |
Feb 08, 2016 |
| Contact name |
Leona Dance Scanlan |
| E-mail(s) |
scanlan.leona@gmail.com
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| Phone |
5305750595
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| Organization name |
NIST
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| Street address |
100 Bureau Dr
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| City |
Gaithersburg |
| State/province |
MD |
| ZIP/Postal code |
20899 |
| Country |
USA |
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| Platforms (1) |
| GPL16579 |
Agilent-023710 Daphnia magna 8x15k oligonucleotide array [Probe Name version] |
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| Samples (71)
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| Relations |
| BioProject |
PRJNA301365 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE74736_RAW.tar |
51.9 Mb |
(http)(custom) |
TAR (of GPR) |
| GSE74736_log2_values_treatment_v_control.xlsx |
79.2 Kb |
(ftp)(http) |
XLSX |
| Processed data are available on Series record |
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