|
| Status |
Public on Nov 06, 2015 |
| Title |
FM550 0.0486 N1 |
| Sample type |
RNA |
| |
|
| Source name |
FM550 exposed 0.0486 ng/L
|
| Organism |
Daphnia magna |
| Characteristics |
tissue: whole organism
developmental stage: adult
age: 2 weeks old
|
| Treatment protocol |
Exposed to 1/10 LC50 concentrations of FR or to solvent control. Also exposed to four additional FM500 concentrations.
|
| Growth protocol |
Grew daphnids in COMBO growth medium. Fed and changed media three times per week; 8 hours dark and 16 hours light per day.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Phenol and chloroform extraction (Trizol) with handheld homogenizer
|
| Label |
Cy3
|
| Label protocol |
as per Agilent Low-Input Quick-Amp one-color array kit and protocol. 200 ng total RNA starting material.
|
| |
|
| Hybridization protocol |
as per Agilent Low-Input Quick-Amp one-color array kit and protocol.
|
| Scan protocol |
16-bit GenePix 4000B microarray scanner with 5-micron resolution and features, edited with GenePix Pro 6.0
|
| Data processing |
Regression analysis in GenePixPro, subsequent analysis as in Loguinov 2004, with a “Treatment vs. Control” design. Global normalization with loess was applied to the data. The file log2_values_treatment_v_control.xlsx contains the normalized log2 ratios for each treatment vs control, summarized across the replicates.
|
| |
|
| Submission date |
Nov 05, 2015 |
| Last update date |
Nov 06, 2015 |
| Contact name |
Leona Dance Scanlan |
| E-mail(s) |
scanlan.leona@gmail.com
|
| Phone |
5305750595
|
| Organization name |
NIST
|
| Street address |
100 Bureau Dr
|
| City |
Gaithersburg |
| State/province |
MD |
| ZIP/Postal code |
20899 |
| Country |
USA |
| |
|
| Platform ID |
GPL16579 |
| Series (1) |
| GSE74736 |
Gene transcription, metabolite and lipid profiling in eco-indicator daphnia magna indicate diverse mechanisms of toxicity by legacy and emerging flame-retardants. |
|
