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| Status |
Public on Dec 05, 2015 |
| Title |
Differentially expressed genes between WT and AtC3H17-overexpressing transgenic plants |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
We characterized the biological and molecular functions of AtC3H17, a unique Arabidopsis gene encoding a non-tandem CCCH zinc finger protein, in plant development. To investigate the downstream regulatory mechanisms of AtC3H17, whole genome microarray expression profiling was carried out. The experiment was designed to identify differentially expressed genes between WT and AtC3H17-overexpressing transgenic plants (AtC3H17 OXs) grown for 14 days under SD growth conditions. Interestingly, the most up-regulated genes were 12S seed storage globulin genes, CRUCIFERIN A (CRA1) and CRUCIFERIN 3 (CRU3), and seed oil-body protein genes such as OLEOSIN 1 (OLEO1) and OLEO2 in AtC3H17 OXs compared with WT. We performed quantitative RT-PCR and confirmed that transcription levels of CRU3, OLEO1, OLEO2, and 2S seed storage albumin 1 (At2S1) were higher in AtC3H17 OX seedlings than in WT seedlings.
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| Overall design |
Differentially expressed genes between WT and AtC3H17 OX at 14 days after germination under SD conditions (8-h-light and 16-h-dark cycle) were explored in whole genome level. Two AtC3H17 OX samples were compared to WT sample as a control.
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| Contributor(s) |
Woo D, Seok H, Park H, Lee S, Tran HT, Lee E, Nguyen LV, Moon Y |
| Citation(s) |
26858286 |
| |
| Submission date |
Dec 04, 2015 |
| Last update date |
Apr 19, 2017 |
| Contact name |
Yong-Hwan Moon |
| E-mail(s) |
moonyh@pusan.ac.kr
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| Phone |
+82515102592
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| Organization name |
Pusan National University
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| Department |
Molecular Biology
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| Lab |
Plant Molecular Genetics Lab
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| Street address |
Busandaehak-ro 63Beon-gil 2, Geumjeong-gu
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| City |
Busan |
| ZIP/Postal code |
46241 |
| Country |
South Korea |
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| Platforms (1) |
| GPL9020 |
Agilent-021169 Arabidopsis 4 Oligo Microarray (V4) (Feature Number version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA305103 |
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